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低密度脂蛋白胆固醇直接测定法的临床应用评价 被引量:5

Clinical Application Evaluation of the Direct Assay Method for Low Density Lipoprotein Cholesterol
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摘要 评价低密度脂蛋白胆固醇 (LDL -C)直接测定法临床应用的可行性 .方法 :将直接测定法 (选择性抑制 )与化学修饰法和Friedewald公式法进行比较 ,并研究其精密度、特异性、线性范围及干扰因素 .结果 :直接法与化学修饰法相关良好Y =0 94 5X + 0 5 61,r=0 978,n =78.直接法与Friedewald公式法在甘油三酯 (TG) <4 5 3mmol/L时有良好相关性Y =0 983X + 1 0 2 5 ,r =0 994 ,n =78.而在甘油三酯 >4 5 3mmol/L时则相关性较差 ,Y =0 82 7X +4 3 5 6,r =0 72 3 ,n =196.直接法比公式法高 ,差异有显著性意义 (P <0 0 5 ) ,LDL -C浓度在 10 4mmol/L范围内线性良好 .LDL -C浓度为低、中、高值标本的两组样品 ( 1 73 ,3 0 8,6 3 3和 1 94 ,3 0 1,6 87mmol/L)的批内和批间CV %分别为 1 94 % ,1 3 6% ,2 0 1%和 2 0 5 % ,1 78% ,2 93 % ,浓度为 11 3mmol/L的TG不影响LDL -C直接测定法 .结论 :LDL -C直接测定法精密度高 ,特异性好 ,线性范围宽 ,干扰因素少 ,简便快速 ,可自动化分析 。 A feasibility study of clinical application of the direct assay method for low density lipoprotein cholesterol (LDL-C) is discussed here. Method Direct assay was compared with chemistry modify method and Friedewalds formula. We evaluated their precision,speciality, linearity and interfere factor of the three methods. Result There was a good correlation between the method of direct assay and chemistry modify method ( Y=0 945X+0 561, r=0 978,n =78). Good correlation also existed between the direct assay method and Friedewalds formula when TG≤4 53?mmol/L( Y=0 983X+1 025, r=0 994, n=78 ) But poor correlation occurred when TG>4 53?mmo/L ( Y=0 827X+4 356, R=0 723, n=196 ). The result of direct assay was higher than that of formula (P<0 05). There was a good linearity when LDL-C ≤10 4?mmol/L. When the concentration of LDL-C at low (1?073?mmol/L, 1 94?mmol/L),normal (3 08?mmol/L, 3 01?mmol/L) and high (6 33?mmol/L, 6 87?mmol/L), their within-run and CV% in the two groups were 1 94%, 1 36%, 2 01% and 2 05%, 1 78%, 2 93% respectively. Triglycerides at 11 3?mmol/L did not interfere the measurement of LDL-C in the direct assay. Conclusion The direct assay of LDL-C had high precision, good specialty, wide linearity range and a few interfere factors. The method was simple, fast and can be automatically analyzed. It is worth to extend its clinical application.
作者 钱净 杨丽琼
出处 《昆明医学院学报》 2001年第4期45-47,共3页 Journal of Kunming Medical College
关键词 低密度脂蛋白胆固醇 直接测定法 评价 Low density lipoprotein cholesterol Direct assay Evaluation
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