摘要
目的 : 研究微量营养素硒、铬、钒、维生素 E(VE)的联合生物学效应在胰岛素依赖性糖尿病 (IDDM)发病过程中对淋巴细胞白细胞介素 - 4(IL- 4)、白细胞介素 - 1 0 (IL- 1 0 )表达的调节作用及对 IDDM发生发展的影响。方法 : 以链脲佐菌素 (STZ)小剂量、多次注射方法 ,制造大鼠IDDM模型 ,用流式细胞仪检测、比较不同组合给予硒、铬、钒、VE对 IL- 4、IL- 1 0表达调控的影响及与血糖、活性氧产生等指标的关系。结果 : IDDM大鼠外周血和脾脏表达 IL- 4、IL- 1 0淋巴细胞数目总体偏低于非糖尿病大鼠 (P<0 .0 5～ 0 .0 1 )。联合给予一定剂量的硒、铬、钒、VE可不同程度地明显提高 IL- 4、IL - 1 0的表达 (P<0 .0 5～ 0 .0 1 ) ,降低升高的血糖水平 (P<0 .0 1 )和减少组织活性氧的产生 (P<0 .0 5～ 0 .0 1 )。结论 : 微量营养素可通过上调 IL- 4、IL- 1 0的表达减轻胰岛β细胞自身免疫性损害 ,防治 IDDM的发生发展。
Objective: To study the regulation roles of Se,V,Cr and VE in the expression of IL-4 and IL-10 by lymphocytes during the onset and progress of IDDM. Methods: The IDDM model of rat was made with multiple injections of low dose streptozocin. The rats were supplemented with Se,V,Cr and VE. The expression of IL-4 and IL-10 by lymphocytes in peripheral blood as well as spleen were determined by flow cytometer. In addition, reactive oxygen and blood glucose were also measured. Results: In IDDM rats, the overall IL-4 or IL-10 positive lymphocytes in peripheral blood and spleen were lower than the non IDDM rats (P<0.05-0.01). Combined administration of Se?V?Cr and VE significantly raised the expression of IL-4 and IL-10 by lymphocytes(P<0.05-0.01), decreased the blood glucose level, and the production of reactive oxygen was also reduced(P<0.05-0.01). Conclusion: Antioxidant micro-nutrients may protect β cells from autoimmune damage through up-regulation of the production of IL-4 and IL-10,and then retard the development of IDDM.
出处
《营养学报》
CAS
CSCD
北大核心
2001年第4期330-333,共4页
Acta Nutrimenta Sinica
基金
国家自然科学基金 ( No.39470 6 12
39870 6 6 7)
吉林省科委基金 (No.990 5 75 -1)
