摘要
目的 观察低浓度氟和地塞米松对成骨细胞生长因子或其受体表达的影响 ,探讨其对成骨细胞发育、分化的可能机制。方法 体外培养MC3T3 E1成骨细胞 ,利用免疫组化方法检测成骨细胞内血小板衍化生长因子 A(PDGF A)和胰岛素样生长因子 Ⅰ的α受体 (IGF Ⅰ Rα)表达 ,并对其结果作图像分析。结果 氟和地塞米松对PDGF A表达在各个时期都没有影响 ,而在细胞培养早期均促进了IGF Ⅰ Rα表达 ,地塞米松的促进作用尤为明显。结论 低浓度氟和地塞米松均促进幼稚成骨细胞中IGF Ⅰ Rα表达 ,IGF Ⅰ Rα表达增高可能与其对成骨细胞增殖、分化影响有关。
Objective Investigate the expression of growth factors or receptors in osteoblast, to understand the possible mechanism of low dose fluoride and dexamethasone on the development and differentiation of osteoblast.Methods Investigate the expression of platelet-derived growth factors (PDGF-A) and insulin-like growth factors (IGF-I-Rα) in osteoblast cultured in vitro by immunohistochemistry, furthermore, to deal with the results by image analysis.Results There were no significant effects of PDGF-A both of low doses fluoride and dexamethasone treated on the MC3T3-E1 osteoblast during the various stages of the development. Otherwise, the expression of IGF-I-Rα might increase treated with them at the early stage of the cell culture, and dexamethasone did more significantly.Conclusion Low doses of fluoride or dexamethasone may promote the expression of IGF-I-Rα in osteoblast precursors, which maybe contribute to the effects of fluoride and dexamethasone on the proliferation and differentiation of osteoblast.
出处
《上海口腔医学》
CAS
CSCD
2001年第4期336-338,345,共4页
Shanghai Journal of Stomatology
关键词
氟
地塞米松
成骨细胞生长因子
表达
MC3T3-E1
PDGF-A
IGF-I-Rα
Proliferation
Differentiation
