摘要
目的 研究大鼠不同发育阶段视皮层神经元电生理学与形态学特性的关系 ,观察电生理特性和形态变化的同步化程度 ,认识正常视觉发育的细胞内机制。方法 应用脑片膜片钳全细胞记录技术和细胞内标记相结合的方法 ,获得 4～ 2 8d Sprague- Dawleg(SD)大鼠视皮层神经元的细胞内微电极记录 ,再进行细胞内标记和组织学染色。 结果 成功标记 2 3例细胞 ,其中锥体细胞与非锥体细胞在电生理特性上差异有显著性。不同发育阶段视皮层神经元形态学上的成熟度不同。 结论 1视觉发育过程中 ,视皮层锥体细胞和非锥体细胞的电生理学特性反映其参与不同的视皮层神经元回路的整合功能。 2视觉发育可塑性关键期内 ,视皮层神经元形态和电生理特性变化的同步化程度大于视皮层下结构。
Objective To investigate the relationship between electrophysiological and morphological properties of neurons in visual cortex of developing rat, speculate the coincided degree between electrophysiological and morphological change and realize the mechanism of normal visual development. Methods Whole cell patch-clamp recording and intracellular staining were used to acquire cellular microelectrode recording in visual cortex from Sprague-Dawley rats (4~28 days old). The histological process was made. Results The differences of electrical feature between pyramidal cells and non-pyramidal cells were significant. The morphological maturity degree is different in developing visual cortex. Conclusion The different function of pyramidal and non- pyramidal cells in local integrition is reflected by their electrical feature in the process of visual development. In critical period of visual development, the coincision degree of the electrophysiological and morphological change in visual cortex is larger than that in the subcortex constructure.
出处
《中华眼底病杂志》
CAS
CSCD
2001年第4期289-292,共4页
Chinese Journal of Ocular Fundus Diseases
基金
国家自然科学基金资助项目 (39970 2 52
39770 2 58
30 0 70 2 54)
关键词
电生理学
动物实验
视觉发育
中高皮层神经元
细胞内染色
Optic Nerve/physiology
Neurons/physiology
Electrophysiology
Patch-clamp techniques
Biotin
Animal testing alteruatives
