摘要
研究LN患者外周血淋巴细胞 (PBL)在IL - 18刺激下细胞凋亡及凋亡相关基因表达情况。方法 :AnniexinV联合PI染色定量法及免疫荧光染色法 ,分析了 12例活动期和 2 6例非活动期狼疮性肾炎患者PBL在IL - 18刺激培养后凋亡发生率 ,凋亡相关基因bcl- 2和Fas蛋白的表达以及疾病活动性与淋巴细胞凋亡发生率的相关性。结果 :在IL - 18刺激培养作用下 ,活动期LN淋巴细胞凋亡发生率明显增高 (P <0 .0 1) ,而非活动期则无明显区别 (P >0 .0 5 ) ,疾病活动性与体外淋巴细胞凋亡率呈正相关 (P <0 .0 1)。bcl-2表达活动期显著性升高 (P <0 .0 5 ) ,非活动期无显著性差异 (P >0 .0 5 )。Fas表达活动期显著升高 (P <0 .0 0 1) ,非活动期明显升高 (P <0 .0 5 )。结论 :IL - 18可引起LN患者外周血淋巴细胞凋亡率的增高 ,表明IL- 18在体内凋亡或凋亡相关性免疫机制中起着一定的作用。
Objective:To study effect of IL-18 on lymphocyte apoptosis and apoptosis gene expression in the peripheral blood of lupus nephritis patients.Methods:Apoptosis and apoptosis gene expression in lymphocyte were investigated in 12 activeand 26 inactive LN patients by using Anniexin V united PI staining method and immunofluorescence staining method.Results:The lymphocyte apoptosis of active LN stimulated by IL-18 was significant higher than that of control( P <0.01).Inactive LN was not significantly different from the control group ( P >0.05).There was significantly correlation between the lymphocyte apoptosis and SLE disease activity ( P <0.01).Expression of bcl-2 in lymphocyte of active LN was significantly higher compared with control( P <0.05),but in inactive LN it was not different from the control (P>0.05).Expression of Fas in lymphocyte of both active LN and inactive LN was significant higher than that of control(P<0.01).Conclusions:Increase of the lymphocyte apoptosis of LN patient suggest that IL-18 can influence the apoptosis in vivo and immune mechanism of apoptosis.
出处
《中国现代医学杂志》
CAS
CSCD
2001年第10期19-20,22,共3页
China Journal of Modern Medicine
