摘要
目的 通过对鼠脑机械创伤后测定脑皮层及海马CA2 区脑源性神经营养因子 (Brain -de rivedneurotrophicfactor,BDNF)的含量变化说明BDNF是否对脑创伤神经元有保护作用。 方法 建立鼠脑创伤模型 ,取鼠脑用免疫组化方法测定创伤后不同时间BDNF表达含量变化。与设立的假手术对照组相比较 ,并进行组间比较。结果 创伤后鼠脑与假手术组比较 ,创伤组BDNF明显表达 ,时间为伤后 12h ,在 2h为表达高峰期 (P <0 .0 1)。
Objective To interpret BDNF have protective function to injured neuron through measuring expression of BDNF in cerebral cortex and hippocampus CA2 of the mechanically injured rat cerebrum.Methods To establish the model of injured rat ceredrum and measure expression of BDNF in different time with the method of immunihistochemitstry in injured rat cerebrum,to compare with the false operative group and between both two groups.Results Expression of BDNF exist in the injured rat brain,namely,expression of BDNF obveriously increase than the false operative group.From the comparing between two groups,increasing expression time is in 1 hour to 12hour after injury,the peak of expressive BDNF is in 2 hour (P<001).Conclusions BDNF can protect neuron avoid to be injured in the damaged rat brain.
出处
《齐齐哈尔医学院学报》
2001年第7期726-727,共2页
Journal of Qiqihar Medical University
关键词
脑源性神经营养因子
脑创伤
表达
Brain-derived neurotrophic factor Rat Injured brain
