摘要
应用光谱法研究透明质酸(HA)与天青A(AA)的相互作用机理,考察了反应体系中pH和AA/HA摩尔比对相互作用的影响,测得AA-HA复合物吸收光谱产生蓝移效应所需的临界AA/HA摩尔比1.28×103,HA对AA的最大结合数3.22×103,实验值与理论值完全一致,对多糖与生物探针的相互作用机理提出了合理的解释.
The interaction of hyaluronic acid (RA) and azur A (AA) was studied by absorption spectra. The influence of pH and the molar ratio of AA/HA on the spectra was investigated. The critical molar ratio (1.28 x 10(3)) necessary for the hypsochromism of the absorption spectra of AA - HA complex and the maximum binding number (3.22 x 10(3)) were obtained by spectrometry. The maximum binding number is consistent with the theoretical value.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2001年第12期2181-2185,共5页
Acta Chimica Sinica
