摘要
目的 :探讨理想的喉癌生物治疗及联合治疗的新方法。方法 :利用裸鼠喉癌模型 ,通过不同的用药途径和模式观察新型重组人肿瘤坏死因子 (NrhTNF α)治疗喉癌裸鼠的疗效。并在光、电镜下观察NrhTNF α作用后肿瘤组织病理学及超微结构 ,探讨其作用机制。结果 :①喉癌裸鼠经NrhTNF α治疗后出现肿瘤出血、坏死 ,肿瘤缩小、消失 ,生存期延长。②NrhTNF α局部应用的抗肿瘤效果优于全身用药。③NrhTNF α与CTX联合应用具有增强抗瘤作用。④组织病理学及超微结构发现 ,NrhTNF α首先引起细胞质内线粒体、内质网肿胀及整个细胞空泡样变性 ,逐步导致核浓缩、崩解 ,最终致细胞死亡。结论 :NrhTNF α具有抗癌活性高、局部用药或联合用药效果更好 ,毒副作用少等优点 ,该研究为临床喉癌生物治疗及联合治疗提供了实验依据和理论基础。
Objective:We aimed at finding ideal biological treatment and regime for laryngeal carcinoma.Method:Nude mice model bearing laryngeal carcinoma were established using laryngeal carcinoma cell line (HEP 2).New recombinant human tumor necrosis factor (NrhTNF α) and cyclophosphamide were administered locally or systemically, single or in combination. The microstructural and ultra micro structural changes of carcinoma after administration of NrhTNF α were observed under light and electron microscopes.Result:①After treatment, the tumor showed hemorrhage?necrosis?regression, the survival time was prolonged.②More significant antitumor effect was observed when locally administered NrhTNF α than intravenously administered of NrhTNF α.③There is a notable synergistic antitumor effect by the combination use of NrhTNF α and cyclophosphamide.④Microscopy showed that cell changes mostly took place in plasma especially in mitochondria and endoplasmic neticula.The mitochondria were highly bloated. Besides, some nuclei were condensed and fragmented resulted in cell death.Conclusion:These studies provide experimental basis for biological treatment of laryngeal carcinoma.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2001年第12期555-557,共3页
Journal of Clinical Otorhinolaryngology
