摘要
目的 探讨烹调油烟冷凝物对人外周血淋巴细胞DNA的损伤作用。方法 采用单细胞凝胶电泳检测不同浓度的烹调油烟冷凝物处理 6 0min后的人外周血淋巴细胞的DNA单链断裂、双链断裂及DNA -蛋白交联的情况。结果 各剂量的烹调油烟冷凝物均可引起DNA单、双链断裂 (与对照组比较 :P <0 0 5 ) ,10 0 μg/ml及其以上浓度的烹调油烟冷凝物可诱导DNA -蛋白交联的形成 (与对照组比较P <0 0 5 ) ,交联率分别为 2 1 35 %、2 7 2 0 %、34 30 %,并存在着明显的剂量 -反应关系。结论 烹调油烟冷凝物能引起人外周血淋巴细胞DNA的损伤 ,DNA的损伤可能是烹调油烟致细胞恶性转化的重要机制之一。
Objective To study the effects of DNA damages in the human peripheral blood lymphocytes induced by cooking oil fume condenates(COFC).Methods The human peripheral blood lymphocytes were incubated in 37℃ for 60 minutes with different concentration of cooking oil fume condensates.The formation of DNA single-strand breaks(SSB),DNA double-strand breaks(SSB) and DNA-protein cosslinks(DPC) of the cells in various groups were detected with the method of single cell gel-electrophoresis(SCGE).Results Various concentration of COFC within the dose range of the experiment all could induce DNA strand breaks of human peripheral blood lymphocytes.At the concentration of 100μg/ml,200μg/ml and 400 μg/ml,COFC could result in DNA-protein crosslinks of human peripheral blood lymphocytes,and it was obvious that dose-response relationship between DNA damages and COFC.Conclusion COFC could induce DNA damages of human peripheral blood lymphocytes,and it may be potential carinogenic risk to human.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2002年第2期137-138,共2页
Chinese Journal of Public Health
基金
湖南省科委资助项目 (0 2 0 - 962 - 0 2 - 3)
