摘要
目的 :观察高同型半胱氨酸血症 (hyperhomocysteinemia)引起的动脉硬化的特点 ,及补充叶酸、维生素B12对动脉硬化病变的作用。方法 :制备家兔高同型半胱氨酸血症、高同型半胱氨酸血症合并高脂血症的动物模型 ,并补充叶酸、维生素B12 ,以正常家兔为对照 ,分别测定各组同型半胱氨酸 (homocysteine,HCY)、叶酸、维生素B12 及血脂水平 ,观察主动脉病理变化。结果 :6～ 8周后发现给予蛋氨酸与同时给蛋氨酸和胆固醇的动物 ,血浆Hcy均显著升高 [分别为 (4 1.3± 19.9)对 (19.6± 5 .1) μmol·L-1,P <0 .0 5 ;(31.6± 5 .7)对 (18.1± 2 .6 ) μmol·L-1,P <0 .0 1],后者血浆胆固醇及甘油三脂水平亦明显升高。而同时补充叶酸、维生素B12 的动物 ,Hcy水平未见变化 ,叶酸、维生素B12 升高且差异有显著性 [分别为 (30 .3± 12 .0 )对 (13.0± 5 .5 ) μg·L-1,(5 3.1± 2 0 .1)对 (31.5± 6 .5 ) μg·L-1,P<0 .0 5 ]。主动脉切片发现蛋氨酸组及蛋氨酸加胆固醇组血管平滑肌细胞增生 ,内膜面积增加 ,蛋氨酸加胆固醇组还见到脂质沉积、斑块形成。蛋氨酸 +叶酸 +维生素B12 组血管内膜正常 ,与对照组相比差异无显著性。结论 :高同型半胱氨酸血症可引起血管内膜增厚、平滑肌细胞增生等早期动脉硬化改变 ,叶酸。
Objective: To study the pathological changes and characteristics of atherosclerosis induced by hyperhomocysteinemia and hypercholesterolemia, and the effects of folic acid and vitamin B 12 . Methods: Four groups of rabbits were studied: 5 as control subjects received a standard diet; 7 received parenteral methionine; 7 received parenteral methionine and folic acid and vitamin B 12 ; 6 received both parenteral methionine and cholesterol rich diet. After being raised for 6～8 weeks, all rabbits were sacrificed. Results: The rabbits that received methionine developed hyperhomocysteinemia (41.3±19.9 μmol·L -1 vs. 19.6±5.1 μmol·L -1 , P <0.05) and arterial lesions with smooth muscle cell hyperplasia and intimal thickening. The rabbits that received both methionine and cholesterol rich diet developed hyperhomocysteinemia (31.6±5.7 μmol·L -1 vs 18.1±2.6 μmol·L -1 , P <0.01), and hypercholesterolemia, and arterial lesions with smooth muscle cell hyperplasia, intimal thickening, elastic lamina disturbance and fatty plaques. The rabbits that received methionine + folic acid + vitamin B 12 displayed no arterial lesions, and had normal homocysteine concentrations and increased folic acid concentrations and vitamin B 12 concentrations (30.3±12.0 μg·L -1 vs 13.0±5.5 μg·L -1 ,53.1±20.1 μg·L -1 vs 31.5±6.5 μg·L -1 respectively, P <0.05). Conclusion: In this rabbit model, hyperhomocysteinemia can induce arterial lesions with early atherosclerosis. Furthermore, folic acid and vitamin B 12 are effective in lowering homocysteine concentration and preventing the pathological changes induced by hyperhomocysteinemia.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2001年第6期536-539,共4页
Journal of Peking University:Health Sciences
基金
卫生部科研基金 ( 98 1 2 5 1)~~
关键词
动脉硬化
病理学
高同型半脱氨酸血症
维生素B12
动物实验
Homocysteine
Arteriosclerosis/etiol
Folic acid/theruse
Hyperhomocysteinemia
Vitamin B 12 /theruse
