摘要
建立了复方鱼腥草片中黄芩苷的 HPL C测定方法。采用 C1 8柱 (2 5 0× 4.6 mm,5 μm) ,甲醇 - 0 .4%磷酸溶液 (5 3∶ 47)为流动相 ,检测波长为 2 77nm。黄芩苷在 10～ 10 0μg/ ml浓度范围内呈良好的线性关系 ,r=0 .9999。平均回收率为 10 0 .6 % ,RSD为 1.6 0 %。
A HPLC method for the determination of baicalin in compound herba houttuyniae ta blets was established. A Hypersil ODS C 18 column(250×4.6 mm,5 μm) was us ed, with the mobile phase of methanol-0.4% phosphoric acid solution(53∶47). Th e detection wavelength was 277 nm. The calibration curve was linear in the range of 10~100 μg/ml( r =0.9999), and the average recovery of baicalin was 100.6 %, RSD was 1.60%.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2001年第11期510-511,共2页
Chinese Journal of Pharmaceuticals
