摘要
目的 :观察海藻硫酸多糖 (SPS)对正常小鼠和免疫低下小鼠免疫功能的调节作用。方法 :测定小鼠腹腔巨噬细胞吞噬鸡红细胞的能力、碳粒廓清能力、血清溶血素形成 ,并进行二硝基氟苯诱导小鼠迟发性变态反应实验、T ,B淋巴细胞增殖实验 ,考察SPS对正常小鼠免疫功能的影响 ;测定环磷酰胺致免疫低下小鼠的血象、胸腺指数和T淋巴细胞亚群 ,进行T ,B淋巴细胞增殖实验 ,考察SPS对免疫低下小鼠免疫功能的影响。结果 :SPS能增强正常小鼠腹腔巨噬细胞吞噬鸡红细胞的能力 ,高剂量SPS能增强小鼠碳粒廓清能力 ,高、中剂量SPS能提高小鼠血细胞凝集程度 ,SPS体外对T ,B淋巴细胞有促增殖作用。对于环磷酰胺所致免疫低下小鼠 ,SPS可以通过调节血象、增加胸腺指数、提高T淋巴细胞和CD+ 8细胞数目、促进T ,B淋巴细胞增殖来缓解小鼠免疫功能的低下。结论 :SPS是一种免疫调节剂 ,具有免疫调节和抗病毒作用 ,是新一代抗HIV药物的发展方向。
Objective:To observe the immune regulating effect of SPS in normal mice and mice with immunosuppression.Methods:Phagocytic function of peritoneal macrophage,carbongranular clearance ability,hemolysin production level,DTH induced by DNFB and T,B lymphocyte proliferation in normal mice were determined.The hemogram,thymus index,type distribution of T lymphocyte and proliferation of lymphocyte T and B in mice with immunodepression were determined.Results:In normal mice,SPS can induce the phagocytic function of peritoneal macrophages,promote the carbongranular clearance at high dose,increase hemolysin production at high and medium dose,accelerate the proliferation of T and B lymphocytes and enhance the specific and nonspecific immune function.In mice with immunodepression,SPS can relieve dysimmunity and increase the thymus index,T lymphocytes,CD + 8 cells and stimulate the proliferation of T and B lymphocytes by regulating hemogram.Conclusion:As an immunoregulator and anti viral agent,SPS may be developed into a new kind of anti HIV agent.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2001年第9期670-675,共6页
Chinese Journal of New Drugs
关键词
海藻硫酸多糖
免疫调节
免疫低下
polysaccharides from seaweed(SPS)
immunologic regulation
immunosuppression
