摘要
为建立白木千体细胞胚胎发生及其植株再生的高频率实验系统 ,对聚乙二醇及干化处理的影响进行了系统研究。结果表明 ,在分化培养基中附加 5 0 g/L聚乙二醇可显著提高愈伤组织的分化频率和每块愈伤组织产生的体细胞胚个数 。
The factors which influenced somatic embryogenesis and plantlet regeneration in Picea meyeri Rehd.et Wils.were studied in order to establish a high frequency embryogenetic system.It was showed that supplementation 50 g/L PEG (Polyethylene glycol) to the differential medium could produce great effects on somatic embryogenesis and the germination frequency of somatic embryos was also improved a lot by desiccation treatment.
出处
《西北植物学报》
CAS
CSCD
2002年第1期74-77,T001,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家"8 6 3"资助项目 ( 86 0 70 5 0 2 )
关键词
白Qian
体细胞胚发生
植株再生
聚乙二醇
干化
Picea meyeri Rehd.et Wils.
somatic embryo
plantlet regeneration
PEG
desiccation
