摘要
根据GenBank中已发表的猫细小病毒基因组中的保守序列 ,利用Goldkey软件设计了一对能扩增 750bp片段大小的引物 ,对某动物园 1只病死东北虎的脾脏样品进行了PCR检测。结果得到了与设计大小完全相符且与标准猫细小病毒扩增产物大小一致的特异核酸带 ,同时对犬瘟热、犬腺病毒、轮状病毒的核酸扩增结果均为阴性。敏感性试验表明 ,此法可检出血凝价为 1 2 8×脾脏匀浆液上清 1 0 - 5倍稀释的模板 ,远高于血凝试验的敏感性 ,为虎、狮。
The conservative domain of genome of feline panleukopenia virus(FPV)reported and registered in GenBank was analyzed and selected for PCR amplification by DNASIS software.One pair of primers were designed with Goldkey system,which can amplify 750bp fragment from the standard FPV and a spleen of Northeastern tiger probably infected FPV.The specific experiment of PCR showed that the primers could only amplify the genome of FPV.No any gene fragments had been amplified from the cells that were infected by canine distemper virus,canine adenovirus,rotavirus.The sensitivy of this experiment showed that the method could amplify the specific fragment from the soliquoid of spleen cell infected by FPV at 10 -5 dilution,which was more sensitive than normal HI.It showed that this study may provide an effective means for clinical rapid diagnosis of FPV in wild animals such as tiger,lion and panda.
出处
《畜牧与兽医》
北大核心
2001年第5期14-16,共3页
Animal Husbandry & Veterinary Medicine
