摘要
目的 克隆血管生成抑制因子Arresten基因 ,测定并分析其基因序列。方法 从人胎盘组织中提取总RNA ,经逆转录 聚合酶链式反应 (RT PCR)扩增出Arresten基因 ;采用T A突出端克隆 ,构建克隆载体 pGEM Arr ,测定Arresten基因序列。 结果 Arresten基因的克隆中 ,以特异引物Pr2为反转录引物的Arresten基因的扩增比用OligodT的效果好。其序列测定证实Arresten基因克隆成功。结论 Arresten基因需用适当方法克隆 。
Objective To clone human arresten gene,sequence and analyze its coding sequence.Methods The total RNA was isolated from human placenta.The arresten gene was synthesized and amplified from the total RNA by RT PCR.The cloning vector pGEM Arr was constructed by means of T A extension cloning and the gene was sequenced.Results Human arresten gene was successfully cloned and sequenced.The coding sequence obtained was correct.Conclusion The successful clone of human arresten gene lays the foundation for the further studying its anti angiogenic activity.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2002年第1期46-47,共2页
Chinese Journal of Experimental Surgery
