摘要
目的 探讨醋酸铅对大鼠脑细胞凋亡的诱发作用及对bcl 2、bax基因表达的影响。方法 2 4只SD大鼠腹腔注射醋酸铅 5d ,剂量分别为 2 5、5 0、10 0mg/kg体重 ,第 6天取其大脑皮层、海马、小脑组织 ,用流式细胞仪分别测定其细胞凋亡率和bcl 2、bax基因的表达产物Bcl 2蛋白、Bax蛋白。结果 各染铅组大鼠皮层、海马、小脑细胞凋亡率明显升高 ,与对照组相比差异具有显著性 ,并具有良好的剂量 反应关系 ;bcl 2基因表达 (FI指数 )明显降低 ,而bax基因表达则明显增加 ,与对照组相比差异均具有显著性 (P <0 0 1) ,并具有良好的剂量 反应关系。Bcl 2 /Bax明显下降 ,与对照组相比差异具有显著性 ,并具有良好的剂量 反应关系。相关分析表明 ,大鼠大脑皮层、海马和小脑细胞凋亡率与Bcl 2含量呈负相关 ,而与Bax含量呈正相关 ,与Bcl 2 /Bax呈负相关。结论 铅可以诱发大鼠脑细胞凋亡 ,其机制可能与脑组织内bcl 2基因表达下调 ,bax基因表达上调以及Bcl 2 /Bax下降有关。
Objectives To explore the effect of lead acetate on the apoptosis of rat brain neural cells and the relationship between the apoptosis and the bcl 2 as well as bax gene expression Methods Lead acetate was given to SD rats by intraperitoneal injection for 5 days at the dosage of 25,50 and l00 mg/kg body weight respectively The rates of apoptosis and the expression of bcl 2 (Bcl 2) and bax (Bax) in neural cells from cerebral cortex, hippocampus and carebellum were measured respectively by flow cytometry (FCM) Results The rates of apoptosis in neural cells from cerebral cortex, hippocampus and cerebellum in every treatment group were significantly higher than that of control ( P <0 01),and there was a significant dose response relationship ( r =0 998,0 989 and 0.997 respectively) The expression of bcl 2 was significantly decreased, whereas bax was significantly increased, in neural cells from cerebral cortex,hippocampus and cerebellum in every lead acetate treatment group (FI) compared with the control group, and there was a significant dose response relationship( r =-0 886,-0 787 and -0 832 respectively for bcl 2, r =0 971,0 988 and 0 991 respectively for bax) The value of Bcl 2/Bax in every treatment group decreased significantly compared with control, and there was a nice dose response relationship ( r =-0 863, -0 829 and -0 999,respectively) Correlation analysis showed that rates of apoptosis were inversely correlated with the expression of bcl 2( r =-0 750,-0 509,and -0 667,respectively),whereas positively correlated with the expression of bax ( r =0 748,0 56l,and 0 668,respectively).And there were inverse correlations between the rates of apoptosis and Bcl 2/Bax expression Conclusion Lead may induce apoptosis in rat brain neural cells through the down regulation of bcl 2 and the up regulation of bax gene expression
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2002年第1期30-33,共4页
Chinese Journal of Preventive Medicine
