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辐射致大鼠平滑肌细胞HPRT基因第7/8外显子突变分析

Analysis of mutation induced by radiation in HPRT gene exon 7/8 of rat smooth muscles cells
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摘要 目的 探讨辐射剂量与平滑肌细胞HPRT基因变异的关系 ,提供放射预防血管再狭窄的基础实验依据。方法 不同剂量的放射性核素1 88Re内辐射平滑肌细胞后 ,应用 6 TG筛选分离HPRT变异细胞 ,采用PCR SSCP技术进行HPRT基因 7 8外显子的变异分析。结果 辐射后平滑肌细胞HPRT基因突变率为 (5 5~ 13)× 10 - 6 ;91株HPRT突变细胞克隆中 ,14 3%呈现 7 8外显子缺失 ,16 5 %呈现点突变 ,7 8外显子总变异率为 30 8% ;辐射剂量与HPRT基因突变率、第 7 8外显子的总变异率及外显子缺失率呈正相关。结论 辐射所致DNA分子的损伤 ,包括基因片段的缺失。 Objective\ To investigate the relationship between radiation dose and HPRT gene locus mutation in rat smooth muscle cells,and provide a molecular basis for prevention of blood vessel restenosis after PTCA. Methods\ The smooth muscle cells cultured in vitro were irradiated by radionuclide 188 Re with different doses.HPRT gene mutation colonies were selected and isolated by 6\|thioguanine.Analysis of mutation in exon 7/8 of HPRT gene were accomplished by polymerase chain reaction and single\|strand conformation polymorphism. Results\ The HPRT gene mutation frequency of rat smooth muscle cells that were irradiated by radionuclide 188 Re ranged from 5 5×10 -6 to 13×10 -6 . Of 91 HPRT gene mutation colonies,13 contained exon 7/8 deletion and 15 had point mutation.The exon 7/8 mutation frequency was 30 8%.There was significant relationship between radiation dose and mutation frequency of HPRT gene and exon 7/8. Conclusions\ The DNA damage and gene mutation induced by radiation was the basis of proliferation inhibition and apoptosis of smooth muscle cells.
出处 《中华放射医学与防护杂志》 CAS CSCD 北大核心 2001年第5期328-331,共4页 Chinese Journal of Radiological Medicine and Protection
基金 国家自然科学基金资助项目 ( 39970 2 2 1)
关键词 基因突变 HPRT PCR-SSCP 鳞188 平滑肌细胞 辐射损伤 辐射剂量 Ionizing Gene mutation 188 Re HPRT\ PCR\|SSCP
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参考文献3

  • 1Leonhardt E A,Mutat Res,1999年,426卷,23页
  • 2Wu H,Int J Radiat Biol,1998年,73卷,149页
  • 3Elizabeth Y,Blood,1996年,88卷,386页

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