摘要
采用高效液相色谱法,以ZorbaxC18 为固定相,水为流动相,示差折光仪为检测器,外标法进行定量分析 ,一次进样可同时测定葡萄糖、异麦芽糖、异麦芽三糖和4_α_葡糖基麦芽糖、异麦芽四糖等,测定了用双酶协同作用制取异麦芽低聚糖工艺产品中的成分,二次确认试验所得结果良好,相对标准偏差为0.28 %～1.7 %(n=5),线性相关系数为0.9996～1.000,低聚糖的最小检测量在微克级。
An analytical method for the determination of constituents of isomaltooligosaccharide is reported by high performance liquid chromatography using Zorbax C18 as a stationary phase, water as a mobile phase and differential refractometer as a detector. Glucose, isomaltose, isomaltotriose and panose, isomaltotetraose etc can be simultaneously determined in a run. The quantity of the constituents was determined by external standard method. The method has been satisfactorily used to determine isomaltooligosaccharides in oligosaccharide production with RSD of 0.28%～1.7%(n=5), linear correlation coefficients of 0.999 6～1.000 and lowest detectable limits of microgram level.
出处
《分析测试学报》
CAS
CSCD
北大核心
2002年第1期63-65,共3页
Journal of Instrumental Analysis
基金
福建省科委重点工业研究项目 (1996Z -113)
