摘要
目的 探讨老年 2型糖尿病 (DM)患者周围神经病变 (DPN)与血清抗神经节苷脂 (GS)抗体之间的关系。 方法 受试者分为 3组 ,DPN组 32例 ,DM组 2 5例 ,对照组 30例。采用美国NicoletVikingⅣ型肌电图仪测定神经传导速度 ,固相酶免法测定各组抗GSIgM抗体、抗GSIgG抗体 ,同时测定白细胞介素 1β(IL 1β)、白细胞介素 2 (IL 2 )、肿瘤坏死因子 (TNF α)、糖化血红蛋白 (HbA1C)、一氧化氮 (NO)。 结果 DPN组患者抗GSIgM抗体、抗GSIgG抗体的阳性率分别为 6 5 6 %和 34 4% ,显著高于DM组及对照组 (P <0 0 1)。DPN组抗GSIgM抗体与糖尿病神经病变临床分级 (DPNC)、HbA1C、IL 1β、TNF α和NO呈显著正相关 ,而与病程及IL 2呈显著负相关 ;抗GSIgG抗体与DPNC呈显著正相关。逐步回归分析 ,对抗GSIgM抗体有显著作用的因素依次为DPNC、NO、IL 1β ,对抗GSIgG抗体有显著作用的因素为DPNC。 结论 抗GS抗体的检测可作为了解DPN自身免疫介导损伤的一项免疫学指标 ,对DPN的诊断及病情的判断有参考价值。
Objective To characterize the relationship between diabetic peripheral neuropathy(DPN) and serum anti gangliosides antibody(GS). Methods Elderly patients were divided into three groups: patients with type 2 diabetes mellitus(DM) showing no DPN,DM group, 25; DPN group, 32; healthy control group, 30. The nerve conduction velocity was measured using Nicolet Viking type IV electromyography(USA). The anti GSIgM Ab and anti GSIgG Ab were examined by solid enzyme immunoassay, and interleukin 1β(IL 1β), IL 2,tumor necrosis factor α(TNF α), HbA 1C and NO were also measured. Results The positive rate of anti GSIgM Ab and anti GSIgG Ab in DPN group being 65.6% and 34.4% respectively, was obviously higher than those in N group and DM group(P<0.01 and P<0.001, respectively). There was a significantly positive correlation between the anti GSIgM Ab and DPN clinical classification(DPNC), HbA 1C , IL 1β, TNF α and NO, and negative correlation with the course of the diseases and IL 2. The anti GSIgG Ab level was positively correlated with the DPNC. Multiple regression analysis showed that DPNC, NO, and IL 1βwere influent factors to the anti GSIgM Ab, and DPNC was significant influent factor to the anti GSIgG Ab. Conclusions The measurement of the anti GS ab might be valuable for the diagnosis of DPN and the judgement of clinical conditions.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2001年第5期352-354,共3页
Chinese Journal of Geriatrics
基金
广西自然科学基金资助项目 (98110 42 )
