摘要
目的 研究角膜基质细胞与ＰＧＡ亲和力，为组织工程技术构建角膜提供重要的理论依据和参数。方法体外分离获得角膜基质细胞，经培养扩增后接种于ＰＧＡ，并对细胞－生物材料复合物进行体外培养，观察细胞生长代谢。应用ＭＴＴ技术测定角膜基质细胞与ＰＧＡ的黏附率。结果 角膜基质细胞能够在ＰＧＡ中黏附、伸展和分泌基质，ＭＴＴ测得细胞黏附率为 ７１．４０％。结论 角膜基质细胞与 ＰＧＡ具有较好的亲和力，ＰＧＡ可以作为构建角膜的生物材料。
Objective To study the affinity of corneal stromal cells and PGA (polyglycolic acid), in order to fabricate a model of cells culture in a three dimensional environment. Methods Approximately 1 million cultured corneal stromal cells were seeded onto the three-dimensional PGA fiber scaffolds. At the 4th day after cell seeding, MTT automated colormetric microassay was adopted to count the number of the cells attached to the PGA fibers. And at the 6th and 18th days, the complexes were observed by a scan electron microscopy(SEM). Result Corneal stromal cells secreted the extracellular matrix(ECM) and multiplied shortly after they were adhered to the PGA. The cell adhersion rate was 71.40%. Conclusion PGA can be fabricated into a suitable three-dimensional scaffold for corneal stromal cells to live in. This cell-polymer complex can be used for tissue engineered cornea research.
出处
《眼科研究》
CSCD
北大核心
2001年第4期308-311,共4页
Chinese Ophthalmic Research
