摘要
目的 观察不同 p H值低温肺保护液对体外循环下未成熟肺急性肺损伤的保护作用 ,探求最适宜 p H值的低温肺保护液 .方法 实验用幼犬 (<30 d) 36只 ,随机分为六组 :A组 :单纯体外循环组 ,B至 F组分别用 p H=6 .6 ,7.0 ,7.4,7.8,8.2肺保护液进行肺动脉灌注 ,阻断 6 0 min,再灌注 6 0min后 ,取标本 ,分别采用髓过氧化酶法、硫代巴比妥酸法和硝酸还原酶法测定 MPO,MDA和 NO,测定肺组织含水量和进行肺组织超微结构观察 .结果 C,D,E,F组的 MPO和MDA均较 A组明显降低 (P<0 .0 5 ,P<0 .0 1,P<0 .0 1,P<0 .0 5 ) ,其中 E组最低 ,NO含量明显上升 ,(P<0 .0 5 ,P<0 .0 1,P<0 .0 1,P<0 .0 5 ) ,肺组织含水量降低 ,其中 D,E组相差明显 (P<0 .0 5 ,P<0 .0 5 ) ,同时发现灌注组的 型上皮细胞、肺血管内皮细胞损伤减轻 .结论 低温肺保护液可减轻体外循环下未成熟肺损伤 ,其中 p H7.8的肺保护液 。
AIM To observe the effect of hypothermic lung preservation solution with different pH on the acute lung injury of immature dog during cardiopulmonary bypass (CPB). METHODS Thirty six experimental infant dogs (30 d) were randomly divided into 6 groups. Group A was CPB con trol group, and pulmonary artery was perfused with lung preservation soluion at pH 6.6, 7.0, 7.4, 7.8, 8.2 in group B to group F respectively. The pulmonary artery was clarped for 60 min and then reperfused for 60 min. The samples were collected, and MPO, MDA and NO were determined by myeloperoxidase method, thiobarbituric acid method and nitrate reductase method respectively. The water content of lung tissue was measured and the ultrastructure of lung tissue was observed. RESULTS MPO and MDA were more lower in group C, D, E, F than in group A ( P <0.05, P <0.01, P <0.01, P <0.05). MPO and MDA in group E were the lowest. However, No in group B to F were significantly higher than that in group A ( P <0.05, P <0.01, P <0.01, P <0.05). The water contents of lung in group D and E were significantly lower than that in group A ( P <0.05, P < 0.05). At the same time,the injuries of type Ⅱ epithelial cells and endothelial cells in the lung blood vessel were attenuated in the perfusion groups. CONCLUSION Hypothermic preservation solution can attenuate the acute lung injury of immature dog during PCB, and the optimal pH is 7.8.
出处
《第四军医大学学报》
北大核心
2002年第2期130-132,共3页
Journal of the Fourth Military Medical University
关键词
体外循环
肺保护液
再灌注损伤
氢离子浓度
extracorporeal circulation
lung preservation solution
reperfusion injury
hydrogen ion concentration
