摘要
目的:研究食管癌患者免疫功能的变化规律,探索食管癌的免疫发病机制。方法:应用形态学方法测定食管癌患者LBT、RBC-C3bRR、RBC-ICR及NTER;用ELISA法测定食管癌患者血清sIL-2R和TNF-α水平;用生化方法测定食管癌患者血清NO含量,并进行统计学分析。结果:食管癌患者血清NO含量、LBT、RBC-C3bRR和NTER均显著低于正常对照组(P均<0.001);而血清sIL-2R含量、血清TNF-α含量及RBC-ICR均显著高于正常对照组(P均<0.001)。肿瘤根治术后患者血清NO含量、LBT、RBC-C3bRR和NTER较术前明显升高(P<0.05;<0.001;<0.001;<0.05),但仍低于正常对照组(P均<0.001):而血清sIL-2R含量、血清TNF-α含量及RBC-ICR较术前明显降低(P均<0.001),但仍高于正常对照组(P均<0.001)。本实验还发现食管癌患者RBC-C3bRR与血清NO含量及LBT呈正相关,与血清sIL-2R含量呈负相关。结论:食管癌患者的多项免疫指标均发生了明显的改变,多项免疫指标的联合检测对食管癌发病机理探讨、疗效观察和预后判断均有重要的临床意义。
Objective:To study the changes in the immune functions of esophageal cancer patients and the immune etiopathology in esophageal cancer.Meth ods:The LBT,RBC-C3bRR,RBC-ICR and NTER in esophageal cancer patients were detected using morphological method.The content of the serum sIL-2R and TNF-αwas detected using ELISA method;the serum NO content was detected using bio-chemical method and analysed statistically.Results:The serum NO content,LBT,RBC-C3bRR and NTER were all lower than that of controls(P<0.001),the content of the serum sIL-2R and TNF-α,RBC-ICR were significantly night than that of controls(P<0.001).mean while,14samples collected from the patients on the tenth day or nearby before and after removal of the cancer were detected for comparison study.The result showed that after removal of the cancer,the serum NO content,LBT,RBC-C3bRR and NTER were significantly higher than that of the patients before operation(P<0.05;<0.001;<0.001;<0.05respectively),but still lower than that of the controls(all P<0.001);meanwhile,the serum sIL-2R and TNF-αcontent and RBC-ICR were significantly lower than that of patients before operation(P<0.001in all of them),but still higher than that of controls(P<0.001in all of them).It was also found that the RBC-C3bRR had a positive relativity with blood serum NO content and LBT,the RBC- C3bRR had a negative rela tivity with serum sIL-2R content of the patients.Conclu sion:The sev-eral immune parameters in esophageal cancer patients are obviously changed,the combination detection of the im mune parameters contribute significance of studying the etiopathologenesis and therapeutic efficacy observation and prog nosis judgement in clinics.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2002年第2期87-90,94,共5页
Chinese Journal of Clinical Oncology
基金
宁夏回族自治区教委科研基金资助
