摘要
目的 :探讨FL、rIL 6、rIL 6R对CD34+ 细胞的体外分化增殖作用。方法 :用磁性细胞分离仪富集人脐血CD34+ 细胞。加入FL、rIL 6、rIL 6R及其不同组合因子 ,不同时间取样计数CD34+ 细胞集落数及细胞数。结果 :FL、rIL 6、rIL 6R扩增细胞形成集落的主要成分为CFU GM ,但在rIL 6 +rIL 6R及FL +rIL 6 +rIL 6R作用下 ,也可形成一定数量的BFU E及CFU M ,而CFU MK则未见形成。FL +rIL 6 +rIL 6R可刺激红细胞生成。可使CD34+ 细胞总数在第 7天时扩增 6 4倍。结论 :FL +rIL 6 +rIL 6R可扩增脐血CD34+ 细胞数量并可促进其分化。
Objective:To determine the effects of FL,rIL 6,rIL 6R in vitro expansion and differentiation of human cord blood CD34 + cells.Methods:Human cord blood CD34 + cells were enriched by magnetic cell isolation system CD34 + cells were incubated in the presence of FL,rIL 6,rIL 6R and their various companision.The total number of cells and colonies were counted at various time intervals.Results:The expanded cells by FL,rIL 6,rIL 6R showed colonies were CFU GM mainly but rIL 6+rIL 6R and FL+rIL 6+rIL 6R,showed some BFU E and CFU Mix but no CFU MK.Erythrocyte cells were conformed and total cells were increased 6.4 folds by FL+rIL 6+rIL 6R.Conclusion:Human cord blood CD34 + cells could expanded and differentiated by FL+rIL 6+rIL 6R.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2001年第8期436-437,共2页
Chinese Journal of Immunology
