摘要
采用了改良K8p和Ay3两种培养基,对天蓝苜蓿进行单细胞培养并得到再生植株。来自天蓝苜蓿胚轴愈伤组织的细胞系,在单细胞培养中的愈伤组织分化率明显高于来自子叶愈伤组织的细胞系。改良K8p培养基较之Ay3培养基更利于天蓝苜蓿单细胞培养。生物素、泛酸钙和葡萄糖对细胞系的细胞分裂、愈伤组织诱导和分化有促进作用。赤霉素促进再生幼芽向植株发育。
Calli were initiated from explants of cotyledon and plumular axis of Medicago lupulina on Ayl medium. Two cell lines were obtained from the ealli of cotyledon and plumular axis in liquid Ay2 medium on a rotary shaker at 120 rpm. Both cell lines were able to form calli through monocell cultures in liquid Ay3 or modified K8p medium. After the further growth of calli on solid Ay3 medium they were transferred onto Af medium for the the plantlet regeneration. Regenerated plantlets were cultured, on As medium and root formation was induced. Cell lines derived from calli of plumular axis had a higher cell density and regeneration frequency than that derived from calli of cotyledon. In contrast to Ay3 medium, modified K8p medium was more favourable for monocell cultures of Medicago lupulina. Biotin. calcium pantothenate and glucose promoted cell division, callus formation and differentiation. GA3 promoted the development of regenerated buds into plantlets.
出处
《实验生物学报》
CSCD
1991年第2期119-125,共7页
Acta Biologiae Experimentalis Sinica
关键词
天蓝苜蓿
单细胞
植标再生
Medicago lupulina. Monocell. Piant regeneration.
