摘要
用非水毛细管电泳电化学检测法分离检测了日夜百服宁中的有效成分 ,研究了电极电位、不同浓度的甲酰胺 (FA)、电解液浓度和酸度、电泳电压及进样时间对电泳分离的影响 ,得到了较为优化的测定条件 .实验结果表明 ,在 2 5 mmol/ L Tris-2 5 mmol/ L H3BO3(表观 p H=8.5 )运行介质中 ,日夜百服宁中的 4种有效成分即扑热息痛 (AP)、盐酸伪麻黄碱 (PH)、氢溴酸右美沙芬 (DM)和扑尔敏 (CM)在 1 2 min内完全分离 .检测电位为 +0 .9V(vs.SCE) .线性范围分别为 AP0 .5～ 2 0 0 mg/ L;PH0 .8～ 3 0 0 mg/ L;DM2 .5～ 3 5 0mg/ L;CM0 .5～ 3 3 0 mg/ L;检测限分别为 AP0 .1 mg/ L;PH0 .5 5 mg/ L;DM1 mg/ L;CM0 .2 mg/
In formamide(FA) medium, the separation and determination of the effective components in Riyebaifuning tablet by nonaqueous capillary electrophoresis with electrochemical detection in the presence of Tris\|H\-3BO\-3 as the background electrolyte were reported. The effect of potential of working electrode, different concentrations of FA, pH, the concentrations of background electrolyte, operation voltage and injection time on capillary electrophoresis were discussed. At the detection potential of \{0\^9 V(\%vs\%. SCE)\}, acetaminophen(AP), pseudoephedrine hydrochloride(PH), dextromethorphan hydrobromide(DM), chlorphenamine maleate(CM) were separated within 12 min by using 25 mmol Tris\|25 mmol H\-3BO\-3(pH=8.5) as a carrier electrolyte at a positive voltage of 27 kV. There are an excellent linearity between peak current and concentration of analytes in the concentration range of AP 0.5\_200 mg/L; PH 0.8\_300 mg/L; DM 2.5\_350 mg/L; CM 0.5\_330 mg/L. The detection limits(\%S/N\%=3) are AP \{0.1 mg/L\}; PH 0.55 mg/L; DM 1 mg/L; CM 0.2 mg/L.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2002年第3期371-375,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 (批准号 :9675 0 3 3 )
广东省自然科学基金 (批准号 :0 0 12 3 7)资助
