摘要
目的 研究神经上皮肿瘤的端粒酶活性及其RNA和人端粒酶逆转录酶 (hTERT)的表达水平 ,为其临床诊断和治疗开拓思路。 方法 改良端粒重复序列扩增法及RT PCR法检测 65例神经上皮肿瘤患者肿瘤标本的端粒酶活性及其RNA和hTERT表达水平 ,并与 8例正常脑组织的标本进行对照。 结果 神经上皮肿瘤端粒酶阳性率为 61 5 % ( 4 0 / 65 ) ,hTERT阳性率为 70 8% ( 4 6/ 65 ) ,两者阳性率及其水平都随肿瘤恶性度增加而升高 (分别r=0 60 7,r=0 678,均P <0 0 1)。 结论端粒酶活性及hTERT与神经上皮肿瘤恶性程度有关 ;hTERT是调节端粒酶活性的关键酶。
Objective To investigate telomerase activity and expression of hTR and hTERT in human neuroepithelial tumors for exploring new strategy for clinical diagnosis and treatment. Methods Telomerase activity was detected by modified TRAP method and the expression of hTR and hTERT was measured by RT PCR method in 65 human neuroepithelial tumors, respectively. Results The positive rates of telomerase and hTERT were 61 5% and 70 8% respectively in human neuroepithelial tumors,and the positive rate and their level of expression were correlated with the degree of malignancy of tumors positively. Conclusions Telomerase activity and hTERT are significantly correlated with the degree of malignancy in human neuroepithelial tumors. hTERT may play a key role in the regulation of telomerase activity.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2002年第2期90-93,共4页
Chinese Journal of Surgery
