摘要
目的 观察缺铁性贫血对大鼠学习记忆能力的影响及其与脑组织中 7种微量元素的关系。方法 应用低铁饮食 (含铁量为 11 9mg/kg的饲料 )建立缺铁性贫血大鼠模型 ,用MG 2型三等分辐射式迷宫检测大鼠的学习记忆能力 ,用三电极等离子体原子发射直读光谱仪测定脑组织中 7种微量元素的含量。结果 缺铁性贫血大鼠在迷宫测验时达标所需反应次数 [(72± 9)次 ]较对照组[(5 0± 9)次 ]显著增加 (P <0 0 1) ;正确反应率 [(5 9± 5 ) % ]较对照组 [(6 6± 9) % ]显著降低 (P <0 0 5 ) ,2 4h后复测仍有相似结果 ,表明缺铁性贫血大鼠学习记忆能力显著降低。脑组织中铁、锌含量[(13 7± 3 5 ) μg/g湿组织、(8 6± 2 1) μg/g湿组织 ]明显低于对照组 [(2 6 1± 2 7) μg/g湿组织、(11 3± 0 9) μg/g湿组织 ,P <0 0 1],锰、镉含量 [(0 5 6± 0 12 ) μg/g湿组织、(0 0 8± 0 0 4) μg/g湿组织 ]明显高于对照组 [(0 46± 0 0 8) μg/g湿组织、(0 0 5± 0 0 1) μg/g湿组织 ,P <0 0 5 ]。 结论 缺铁性贫血大鼠的学习记忆能力降低与其脑组织微量元素的变化有关。
Objective Although anemia is frequently associated with iron deficiency, many studies emphasize numerous non-hematologic manifestations of iron deficiency. Most importantly, there is evidence that iron deficiency has an adverse effect on the brain function. Dietary iron deficiency may create secondary disorders on the absorption, plasma and tissue levels and excretion of other minerals. This study was designed to investigate the effect of iron deficiency anemia on learning and memory ability and its biochemical basis in rats. Methods Thirty-two 4-week-old male Sprague-Dawley rats were randomly divided into two groups, rats in the experimental group were given a diet containing 11.9 mg iron /kg, while rats in the control group were given iron dextran 5 mg per week through intraperitoneal injection. The animals were weighed and blood samples were taken at weekly intervals, from the tail for determination of hemoglobin and free erythrocyte protoporphyrin levels. At the end of week 10, the iron-deficient animals showed a significant reduction of hemoglobin and a significant increase of free erythrocyte protoporphyrin. Then the learning and memory ability was tested by means of MG-2 model maze. The contents of 7 kinds of trace element in brain were determined with the DCP-AES technique. Results The experimental rats needed more training [(72±9) times] than the control animals [(50±9) times, P <0.01] to reach the learning criterion, and the correct reaction rate of the experimental rats was markedly lower than that of the control animals [(66±9)% , P <0.05]. The brain iron (Fe) and zinc (Zn) contents of the experimental group [(13.7±3.5) μg/g wet tissue and (8.6±2.1) μg/g wet tissue, respectively] were markedly lower than those of the control group [(26.1±2.7) μg/g wet tissue and (11.3±0.9) μg/g wet tissue, respectively, P < 0.01]. The manganese (Mn) and cadmium (Cd) contents of the experimental group[(0.56±0.12) μg/g wet tissue and (0.08±0.04) μg/g wet tissue, respectively] were markedly higher than those of the control group [(0.46±0.08) μg/g wet tissue and (0.05±0.01) μg/g wet tissue, respectively, P <0.05]. On the other hand, there were no significant differences in brain selenium (Se), plumbum (Pb), cupreous (Cu) between the two groups. Conclusion The present study suggested that the iron deficiency has an adverse effect on learning and memory ability in rats. This defect may attribute to the abnormal metabolism of trace elements in brain.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2002年第3期161-163,共3页
Chinese Journal of Pediatrics
