摘要
目的 探讨 1,2 5 - (OH) 2 D3 抑制肝癌细胞增殖的机制。方法 体外培养肝癌细胞株SMMC - 772 1,培养基添加 10、5 0及 10 0 (nmol/L) 1,2 5 - (OH) 2 D3 作用 2d、4d、6d后 ,用四唑盐比色试验 (MTT)和平板克隆形成实验检测细胞的存活和生长 ;台盼蓝拒染法绘制生长曲线 ;DNA凝胶电泳检测肝癌细胞凋亡。结果 MTT和平板克隆形成实验检测结果显示 10～ 10 0nmol/L的 1,2 5 - (OH) 2 D3 均对SMMC - 772 1细胞株有显著的抑制作用 ,且呈剂量 -效应关系。DNA凝胶电泳结果显示 1,2 5 - (OH) 2 D3 能够诱导SMMC - 772 1细胞凋亡。结论 1,2 5 - (OH) 2 D3 对于人肝癌细胞株SMMC - 772 1的增殖具有显著的抑制作用 ,其机理可能是通过干扰肝癌细胞的DNA代谢和诱导肝癌细胞凋亡。
Objective To study the mechanisms of inhibitory effects of 1,25 Dihydroxyvitamin D\-3 on hepatocellular cell line SMMC 7721.Methods SMMC-7721 cells were incubated in culture media with 10nmol/L?50nmol/L and 100nmol/L 1,25-Dihydroxyviatmin D 3 for 2d,4d,6d respectively.MTT test,Trypan blue exclusion test and DNA gel electrophoresis were used.Results 1,25-Dihydroxyvitamin D 3(10-100nmol/L)could inhibit the proliferation of hepatocellular cell line SMMC-7721 in dose dependent manner.DAN gel electrophoresis showed that the apoptosis of the hepatocellurlar cells could be induced by 1,25-Dihydroxyvitamin D 3(50nmol/L).Conclusion It was concluded that 1,25-Dihydroxyvitamin D 3 can inhibit proliferation of hepatocellular cells,probably by the interference of DAN metabolism and cell apoptosis.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2002年第3期310-311,共2页
Chinese Journal of Public Health
