摘要
目的 研究丹酚酸 A(Sal A)对人血清低密度脂蛋白 (LDL)氧化修饰的抑制作用。方法 用Cu2 + 体外诱导LDL过氧化 ,观察Sal A对MDA ,脂褐素和维生素E含量 ,氧化LDL电泳迁移率以及对LDL氧化过程中自由基的产生及Sal A螯合Cu2 + 的作用。结果 LDL经Cu2 + 诱导过氧化后 ,丙二醛和脂褐素的生成增加 ,维生素E含量下降 ,LDL电泳迁移速度加快 ,LDL氧化过程中自由基的生成增加。 10 - 6 ～ 10 - 4mol·L- 1 Sal A可剂量依赖性地抑制上述改变 ,并且Sal A能螯合Cu2 + 。结论 丹酚酸 A可有效地抑制Cu2 + 诱导的人LDL氧化 。
AIM Oxidized low density lipoprotein (LDL) is involved in the development of atherosclerosis. Oxidative modulation of serum LDL is related to oxygen free radicals. Antioxidants have beneficial effects on oxidative modulation of LDL and development of atherosclerosis. Salvia miltriorhiza (Danshen) preparations have been widely used in the treatment of cardio cerebral vascular diseases in China. Salvianolic acid A (Sal A), one of the components of Salvia miltriorhiza , was shown to have strong antioxidative activity. The aim of this investigation was to evaluate the effect of Sal A on human LDL oxidative modulation mediated by copper ions. METHODS Oxidation of human LDL was performed in pH 7 4 phosphate buffered saline with 10 μmol·L -1 CuSO 4 at 37℃ water for 20 h. The content of malondialdehyde (MDA), lipofuscin and vitamin E in LDL as well as the rate of eletrophoretic mobility (REM) of LDL were measured. The generation of free radicals during LDL oxidation was detected by low level chemiluminescence (LL CL). The chelation of Cu 2+ by Sal A was detected by UV spectrum scanning. RESULTS Sal A (10 -6 to 10 -4 mol·L -1 ) was shown to markedly reduce the production of MDA and lipofuscin as well as the consumption of vitamin E during LDL oxidation. Sal A (10 -4 mol·L -1 ) was also shown to inhibit the increase of REM of LDL caused by oxidative modification. In addition, the spectrum of LL CL showed that Sal A (10 -6 to 10 -5 mol·L -1 ) decreased the generation of free radicals during LDL oxidation in a dose dependent manner. The differential UV spectrum of Sal A in the presence of Cu 2+ indicated that Sal A could chelate copper ions. CONCLUSION Sal A has inhibitory effect on Cu 2+ mediated human LDL oxidation through chelating Cu 2+ and scavenging free radicals.
出处
《药学学报》
CAS
CSCD
北大核心
2002年第2期81-85,共5页
Acta Pharmaceutica Sinica
基金
科技部经费资助项目 (G2 0 0 0 0 5 70 10 )
