摘要
本文报道了用咖啡因与肝素、钙离子载体(I-A)协同处理诱导马、驴、猪冷冻精子体外获能的研究结果.咖啡因与肝素、I-A均有协同作用.在肝素或I-A处理中添加咖啡因,不仅能提高穿透率,而且能促进卵内雄原核的形成和发育.精子先经洗涤,再用咖啡因与肝素或I-A协同处理,可得到良好的获能效果.
The in vitro capacitation of frozen-thawed spermatozoa of stallion, jackass and boar induced by caffeine in combination with heparin or ionophore A23187(I-A) was conducted in the study. It was shown that caffeine when used with heparin or I-A had a synergetic effect to the penetration rate and male pronucleus formation. After washing the spermatozoa were treated with caffeine and heparin or caffeine and I-A, which could be helpful to increasing the capacitation efficiency.
关键词
家畜
冷冻精子
体外获能
咖啡因
frozen-thawed spermatozoa
in vitro capacitation
caffeine
heparin
ionophore A 23187
farm animal
