摘要
目的 通过对正常孕妇及妊娠高血压综合征 (妊高征 )孕妇胎盘组织中去甲肾上腺素(NE)及多巴胺 (DA)的定位及定量测定 ,探讨其与妊高征发病的关系。方法 应用免疫组织化学 (免疫组化 )链霉素抗生物素 -过氧化物酶法 ,对 31例正常早、中、晚孕妇女 (正常孕妇组 ) ,及 38例轻、中、重度妊高征孕妇 (妊高征孕妇组 )的胎盘组织进行NE和DA定位 ;并应用电子计算机图像分析系统进行定量 ,其结果以灰度值倒数表示。结果 (1)两组孕妇胎盘组织NE及DA免疫组化定位 :正常孕妇组中 ,早期孕妇的胚胎绒毛滋养细胞NE及DA免疫反应呈现强阳性 ,呈深棕色 ,定位于细胞滋养细胞的细胞膜及细胞浆中 ;中、晚期孕妇胎盘组织NE及DA免疫反应表现为阳性 ,呈棕色 ,或弱阳性呈浅棕色 ,定位于合体滋养细胞的细胞膜及细胞浆 ,以及毛细血管内皮。妊高征孕妇组的胎盘组织NE、DA定位与正常孕妇组中的晚期孕妇相同。 (2 )两组孕妇胎盘组织NE及DA免疫组化定量 (灰度倒数值 ) :正常孕妇组NE由孕早期的 1 5 95± 0 0 18降至中、晚期的 1 4 88± 0 0 19和 1 4 19± 0 0 14 (P<0 0 1) ;DA由孕早期的 1 5 5 2± 0 0 6 7,降至中、晚期的 1 36 9± 0 0 5 1和 1 30 2± 0 0 13(P <0 0 1)。妊高征孕妇组妊高征轻、中、重度的DA ,依次为 1
Objective To study the distribution and levels of norepinephrine (NE) and dopamine (DA) in placenta tissues of normal pregnancy and pregnancy induced hypertension syndrome (PIH) and discuss the relationship of NE and DA with PIH Method Control group was consisted of thirty one normal pregnancies divided into three stages (early, middle and late). Study group was consisted of thirty eight women with PIH in mild, moderate and severe degrees. The distribution and levels of NE and DA in the placental tissues were detected by immunohistochemistry (SP) and computer image analysis. Results (1) In the control group, the levels of NE and DA located in the cell membrane and cytoplasm of cytotrophoblast were higher in early pregnancy than that located in the cell membrane and cytoplasm of syncytiotrophoblast and endothelium of capillary vessel in middle and late stages. The results in PIH were same as late stage in control group. (2) The expression of NE (1 595±0 018) and DA (1 552±0 067) were decreased gradually in early pregnancy than that of NE (1 488±0 019, 1 419±0 014, P <0 01) and DA (1 305±0 023,1 237±0 024, P <0 01) in middle and late stages in control group; the expression of DA following with the PIH degrees were decreased (1 378±0 029 vs 1 305±0 023 vs 1 237±0 024, P <0 01); the expression of NE following the PIH degrees were increased (1 494±0 018 vs 1 624±0 014 vs 1 917±0 044, P <0 01). Conclusion These findings suggested that NE and DA located in placental cytotrophoblast in early pregnancy and syncytiotrophoblast in middle and late stages and continuously distributed properly with the gestation. The level of NE increased with degrees PIH.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2002年第3期142-145,W001,共5页
Chinese Journal of Obstetrics and Gynecology
