摘要
目的 观察胎鼠宫内缺血缺氧后 ,线粒体与内质网钙离子 腺苷三磷酸酶 (Ca2 + ATPase)与钠离子 钾离子腺苷三磷酸酶 (Na+ K+ ATPase)的变化 ,及细胞内Ca2 + 超载与紊乱的机理。方法 制备胎鼠宫内不同程度缺血缺氧及再灌注模型 (缺血缺氧组 :缺血缺氧时间分别为 15、30、4 5、6 0min ;再灌注组 :缺血缺氧 15min后 ,分别再灌注 1、4、8、15、2 4h) ,各时间点分别有胎鼠 7～ 11只 ,假手术组胎鼠 12只。分离出胎鼠脑组织中线粒体与内质网 (匀浆后称微粒体 )行酶活性测定。结果 缺血缺氧组 :缺血缺氧 15、30、4 5、6 0min时 ,线粒体Ca2 + ATPase活性明显减弱 ,分别为 (6 1± 0 5 )、(5 7±0 8)、(5 7± 0 5 )、(5 4 1± 0 7) μmolPi/mg(蛋白·小时 ) ,差异有极显著性 (P <0 0 1) ;Na+ K+ ATPase活性也进行性降低 (P <0 0 1)。内质网Ca2 + ATPase活性变化不明显 (P >0 0 5 ) ,而Na+ K+ ATPase活性仍进行性降低 (P <0 0 1)。再灌注组 :1、4、8、15、2 4h时 ,线粒体Ca2 + ATPase活性明显减弱 ,分别为(7 0± 1 0 )、(6 8± 1 2 )、(7 5± 0 8)、(7 2± 0 9)、(6 7± 0 9) μmolPi/mg(蛋白·小时 ) ,差异有极显著性(P <0 0 1) ;内质网Ca2 + ATPase活性变化不明显 (P >0 0 5 ) ;
Objective To investigate the mechanism of intracellular calcium and other ions disturbance by measuring the activity of Ca 2+ adenosine triphosphatase (Ca 2+ ATPase) and Na + K + adenosine triphosphatase (Na + K + ATPase) Methods Model of fetal rats ischemia and reperfusion was established The duration of ischemia was 15,30,45 and 60mins respectively;after ischemia for 15 mins, reperfusion for 1,4,8,15 and 24 hours There were 7 11 fetal rats sacrificed at different time points respectively, 12 rats in sham for control The mitochondria and endoplasmic reticulium (microsomia) were estracted and the activity of the enzyme was measured Results In the ischemia group: with the development of ischemia, the activity of Ca 2+ ATPase in mitochondria decreased gradually ( P <0 01), but there was no obvious changes in endoplasmic reticulum The activity of Na + K + ATPase in the two organells decreased progressively ( P <0 01) While in the reperfusion group: during the 24 hours of reperfusion, the activity of Ca 2+ ATPase in mitochondria decreased obviously ( P <0 01), but no obvious change in endoplasmic reticulum Meanwhile Na + K + ATPase in this two organells increased to normal level after reperfusion, but 8 hours later, it decreased for the second time Conclusion The changes of Ca 2+ ATPase in mitochondria play an important role in the overload of intracellular Ca 2+ during ischemia and reperfusion, but in endoplasmic reticulium it needs further investigation Na + K + ATPase in the two organells may be related with their edema
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2002年第3期146-148,共3页
Chinese Journal of Obstetrics and Gynecology
基金
国家"九五"公关课题基金资助 (96 90 2 0 6 0 4)
关键词
ATP酶
线粒体
内质网
子宫内缺血缺氧
新生儿缺血缺氧性脑损伤
再灌注
动物实验
Fetal anoxia
Cerebral ischemia
Cerebral anoxia
Ca(2+) Transporting ATPase
Na(+) K(+) exchanging ATPase
Mitochondria
Endoplasmic reticulum
