摘要
利用Sanger双脱氧链终止法对黄羽扇豆（Lupinusluteus）翻译延伸因子2（EF2）的全长cDNA克隆进行了序列分析。该cDNA长度为2794bp,其中包括5’末端的80bp非翻译区,3’末端185bp非翻译区和2529bp长编码序列,3’末端为一18bp poly（A）尾。与其它GTP结合蛋白比较其编译的843aa（氨基酸）序列,发现它们具有显著的同源性;黄羽扇豆EF2与甜菜EF2的氨基酸序列90％相同。其差异主要存在于序列的中部;而与肽基tRNA和核糖体作用部位、与GTP结合部位和GTP酶活性有关的部位具有很高的保守性。黄羽扇豆EF2第700个氨基酸残基组氨酸是白喉毒素的ADP-核糖基化部位。
A full-length cDNA clone encoding Lupinus luteus elongation factor 2 (EF2) involved in the elongation step of protein synthesis was sequenced. The cDNA insert contains a 80-bp 5'-untranslated region,a 2529-bp long coding sequence and a 185-bp 3'-untranslated region. The deduced amino acid sequence (843 residues) from this 2794 bp nucleotide sequence shares 90% homology with that from Beta vulgaris. The sequence analysis supports that diphthamide {2-[3-carboxyamido-3-(trimethylammonio) propyl] histidine( , the site of ADP-ribosylation by diphtheria toxin (DT), is produced by post-translational modification of a histidine residue in the primary translational product. Sequence comparision with other EF2s and GTP-binding protein typa shows that the domains, which are likely to be involved in GTP binding and GTPase activity, and interaction with ribosome and toxins,are highly conserved.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第1期102-105,共4页
Scientia Agricultura Sinica
基金
国家留学基金管理委员会资助
