摘要
目的 在对复方红芪提取液的研究中 ,发现其能促进外周神经损伤的修复 ,并发现其有促进许旺细胞增殖的作用。为了进一步探索复方红芪提取液对神经修复影响的机制 ,观察其对许旺细胞中的酪氨酸蛋白激酶 (PTKs)活性的影响。 方法 使用按 1∶160 0 ,1∶32 0 0 ,1∶64 0 0 ,1∶12 80 0稀释的复方红芪提取液 ,培养新鲜切取的SD大鼠坐骨神经。于培养后第 2 4、48和 96h时 ,使用 [Iγ 32 P]ATP掺入、液闪计数法 ,观察许旺细胞中PTKs的活性变化。使用神经生长因子 (NGF)作阳性对照 ,正常培养液作阴性对照。 结果 正常培养条件下 ,离体神经中许旺细胞的PTKs呈无活性状态。神经生长因子组可见PTKs轻度升高 ,至 96h活性仍有活性且较前稍强 ,峰值为 0 15 48。红芪组可见PTKs活性显著增加 ,随后即下降。峰值出现在第 48h ,1∶160 0 ,1∶32 0 0和 1∶64 0 0三种浓度间变化不明显 ,峰值分别为0 44 60、0 332 6和 0 4169,1∶12 80 0浓度时PTKs活性无变化。秩和检验第 48h、1∶64 0 0以上浓度的红芪培养与神经生长因子及正常对照组差异有显著性 (P <0 0 0 5 ) ,以后各时间间差异则无统计学意义。不同浓度的红芪提取液培养不同时间 ,PTKs活性呈负相关变化 ,秩和检验差异有显著性 (P <0 0 0 5 )。 结论 复方红?
Objective To understand the mechanism of extracts of Hedysarum ploybotrys hand Ma22 on Schwann cell,we studied the effects of the extracts on protein tyrosine kinase (PTKs) in vitro Methods Getting sciatic nerve by operation from SD rats and cultured them in PRMI 1640 medium with 15% embryo bovine serum and with Hedysarum Ploybotrys Hand Ma22 at rate of 1∶1 600,1∶3 200,1∶6 400,1∶12 800 or with nerve growth factor (NGF) NGF as positive control and normal medium as negative one After 48,72 and 96 hours cultures,the sciatic nerve been captured separately and used to examine the activity of PTKs by [γ 32 P] ATP incorporation test using protein tyrosine kinase assay system Result In normal culture condition,there are no active PTKs Cultured with NGF and Hedysarum Ploybotrys Hand Ma22,the activity of PTKs increases notable The peak of NGF group appears at 48th hours and 96th hours were 0 0636 and 0 1548 respectively. Hedysarum Ploybotrys Hand Ma22 at 48th hours were 0 4460,0 3326 and 0 4169 at concintration of 1∶1 600,1∶3 200,1∶6 400, and decrease after it. Rank sum test show the correlations to time were negative ( P <0 005) Analysis of variance shows there were significantly difference between the NGF and Hedysarum Ploybotrys Hand Ma22 at 48th hours ( P <0 001) Conclusion Extracts of the Hedysarum Ploybotrys Hand Ma22 can promote the differentiation of Schwann cell by activeate PTKs signal pathway
出处
《中华显微外科杂志》
CSCD
北大核心
2002年第1期38-40,共3页
Chinese Journal of Microsurgery
基金
国家自然科学基金资助课题 (39870 851 )
