摘要
利用RAPD标记从分子水平上对在生产上有较大影响的 5 1个抗枯萎病陆地棉品种进行了遗传多样性分析。 5 1个陆地棉品种在 4 1个具多态性的随机引物上扩增得到 82个多态性位点。应用NTSYS pc 1.80数据分析软件 ,非加权组平均法 (UPGMA)聚类。 5 1个品种之间的平均成对Jaccard’s相似系数为 0 .5 98。有 17.1%的品种对在遗传上相似性较大 ,相似系数大于 0 .70 0。品种对相似系数在平均值附近 [0 .5 0 0 ,0 .70 0 ]区间上所占的比例最大 ,为 6 6 .9%。遗传差异较大 (相似系数小于 0 .5 0 0 )的品种所占的比例仅为 16 %。总体来说 ,我国抗枯萎病棉花品种之间的相似性较高。我国陆地棉品种资源整体上遗传多样性水平低下 ,以及我国抗枯萎病品种资源狭窄的遗传基础是导致陆地棉品种遗传多样性较贫乏的重要因素。从亚洲棉、海岛棉和其它棉属种内引进抗病基因成为棉花抗枯萎病育种取得突破性进展的关键。研究同时以相似系数矩阵为基础 ,构建了 5 1个抗枯萎病陆地棉品种的UPGMA树状聚类图。
Forty one polymorphic decamer primers with clear and repeated band pattern, which were screened out of 300 randomly selected primers, were used to evaluate genetic diversity of cultivars of G. Hirsumtum L. resistance to Fusarium wilt. Fifty one cultivars obtained 82 polymorphic loci. Jaccard's genetic similarity coefficients were calculated using the software of NTSYS pc version 1.80, and a dendrogram was constructed by the unweighted pair group method of arithmetic average (UPGMA). Mean similarity coefficient among cultivars resistance to Fusarium wilt was 0.598. There were about 17.1% of cultivar pairs with pairwise similarity coefficients exceeding 0.700, which had great genetic similarity. Most of cultivar pairs had the value of pairwise similarity coefficients from 0.500 to 0.700, with the percentage of 66.9%. Sixteen per cent of cultivar pairs had better genetic difference, with pairwise similarity coefficients less than 0.500. Generally, there was a low level of genetic diversity among cultivars resistance to Fusarium wilt. The small genetic diversity of the whole Chinese upland cotton might affect the genetic diversity of cultivars resistance to Fusarium wilt. However, poor genetic base of cultivars resistance to Fusarium wilt would be another important factor to the narrow genetic diversity. It might be an active way to attain breakthrough in developing fine disease resistant cultivars by introducing disease resistant genes from exotic species, such as sea island cotton, Asian cotton and relative species of G. hirsutum L.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第3期272-276,共5页
Scientia Agricultura Sinica
基金
国家自然科学基金 (3 9770 472 )
国家转基因植物研究与产业化开发专项 (J0 0 B 0 0 2 7)
