摘要
采用单粒传的方法从大白菜耐热品种 177和热敏感品种 2 76杂交后代获得遗传性稳定的重组近交系群体 ,以此为材料用同工酶以及RAPD和AFLP分子标记技术鉴定了与大白菜耐热性数量性状相关的遗传标记 ,单因子方差分析和多元线性回归分析的结果表明 ,有 9个与耐热性QTL紧密连锁的分子标记 ,包括 5个AFLP标记 ,3个RAPD标记和 1个PGM同工酶标记 ,这些标记对耐热性遗传的贡献率为 4 6 .7%。 9个标记中有 5个分布在同一连锁群上 ,其他4个标记与任何一个标记无连锁关系 ,表明上述 9个标记分布在大白菜的 5个连锁群上。
Genetic stable colony of recombination inbred line population was derived from a cross between a heat tolerance line 177 with a heat sensitive line 276 of Chinese cabbage (Brassica campestris ssp. pekinensis) by single seed generation. The colony was analyzed using isozyme, RAPD and AFLP techniques to determine molecular markers that linked with heat tolerance quantitative trait loci (QTL) of Chinese cabbage. The results of variance analysis of single factor and multiple linear regression analysis indicate that there are 9 molecular markers closely linked with heat tolerance QTL, they are 5 AFLP markers, 3 RAPD markers and 1 PGM isozyme marker. Total genetic contribution rate of these makers for heat tolerance is 46.7%. Five of the nine markers distribute in one linkage group and other 4 markers have no any relation with the markers in the group. The 9 heat tolerance linkage markers distribute in 5 independent locations of gene group in Chinese cabbage.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第3期309-313,共5页
Scientia Agricultura Sinica
基金
北京市重点试验室种质改良项目
