摘要
本文报道了高效液相色谱法测定美索巴莫(片)的含量及其有关物质的方法。采用YWG-C_(18)柱,以甲醇-0.05mol/L磷酸盐缓冲液(pH4.5)(30:70)为流动相,咖啡因为内标物,检测波长274nm。本方法专属性及重现性均好,可用于控制美索巴莫及其片剂的质量。
Objective: Studies on the assay and determination of related substances for methocarbamoland its tablets by HPLC. Method: YWG-C_(18)Column (25cm×4.6mm,10um). The mobile phase is amixtureof consisted of 0.05M phosphate buffer (pH4.5)-methanol (70:30), with the detection wavelength at274nm. Results: The linear correlation of methocarbamol was observed from the concentrations of 0.5～2. 0mg/ml (r=0.9999). The average recovery of methocarbamol was (99.90±0.41)% (n=9). Conclu-sion: The method was convenient,selective and reproducible for assay and determination of related sub-stances of methocarbamol.
出处
《中国药品标准》
CAS
2002年第1期50-52,共3页
Drug Standards of China
