摘要
探讨灵芝中酸性组分的提取、分离及分析方法 ,并研究其抑菌活性 .结果表明 ,灵芝醇提物经碱处理分离出总酸性组分的产率为 0 .8%～ 1.1% ,当以 n- C6 H1 4/ CH2 Cl2 / CHCl3/ Me OH(体积比为 4 .0∶ 3.0∶ 2 .5∶ 0 .5 )为展开剂时 ,薄层色谱仅显示 Rf 值为 0 .5 4～ 0 .12的 6条主要带 (S1 ～ S6 ) ;抑菌圈法测定结果显示 :该灵芝的酸性组分为2 5 m g/ m L时对金黄色葡萄球菌 (G+ )和大肠杆菌 (G- )均有明显抑制作用 ;粗灵芝酸性组分经硅胶柱层析纯化后 ,CHCl3/ Me OH (V∶ V=98∶ 2 )洗脱部分 (S1 )对大肠杆菌有明显抑制作用 ,而对金黄色葡萄球菌呈明显抑制作用的则集中在 CHCl3/ Me OH(V∶ V=5 0∶ 5 0 )洗脱部分 (S5,S6 ) .上述灵芝酸性组分的提取与分离方法简便易行 ,成分与产率稳定可控 ,且有明显抗菌活性 ,在抗菌制剂应用方面有潜在的药用价值 .
Methods of extraction, separation, identification and about the antibacterial activity of acidic components from Ganoderma lucidum are investigated. The results showed that the yield of acidic components was 0 8%～1 1% of dry Ganoderma lucidum , when isolated by alkalinization from alcohol extract. Using n C 6H 14 /CH 2Cl 2/CHCl 3/MeOH(the volume ratio is 4 0∶3 0∶2 5∶0 5) as the mobile phase on silica gel film, the acidic components were identified as six major spots (S 1～S 6), with their R f values ranging from 0 54 to 0 12. The antimicrobial circle test showed that the acidic components at a concentration of 25 mg/mL had obvious action in bacteriostasis upon S.Aureus (G +) and E.coli (G -). Elution with CHCl 3/MeOH( V∶V =98∶2)(S 1) had bacteriostasis on E.coli , while elution with CHCl 3/MeOH( V∶V =50∶50)(S 5,S 6) exhibited bacteriostasis on S.Aureus . The above methods for extraction, separation and identification of acidic components from G.lucidum are simple and efficient. Furthermore, the obtained acidic components are controllable and bacteriostatic, and these may have potential medicinal application in antibacterial reagents.
出处
《北京理工大学学报》
EI
CAS
CSCD
北大核心
2002年第1期125-128,共4页
Transactions of Beijing Institute of Technology
基金
部级基金资助项目 (原国家科委生命科学技术发展中心主任基金 )
关键词
灵芝
酸性组分
薄层色谱
硅胶柱层析
抑菌活性
提取分离
药用价值
Ganoderma lucidum
acidic components
thin layer chromatography
silica gel column chromatography
antibacterial activity
