摘要
为筛选出对刺梨白粉病具有良好抑制作用的拮抗菌,从健康刺梨及其根系土壤进行微生物分离,以刺梨白粉病病原菌(Sphaerotheca pannosa)为靶标菌株,采用叶盘法对拮抗菌进行筛选,通过形态学、生理生化特征以及16S rRNA对筛选的拮抗菌进行鉴定,测定其OD值绘制生长曲线,并探究拮抗菌对病原菌孢子萌发和菌丝的影响及其产酶情况。结果表明:共分离得到48株细菌,其中JK-6和JK-32具有显著抑制作用,经鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)和空气芽孢杆菌(Bacillus aerius),对刺梨白粉病的防治效果达到了60%和57%;拮抗菌作用下致使病原菌的孢子在萌发过程中发生异常膨大现象,菌丝表面变得粗糙、膨大、缠绕和融断;产酶情况表明两株拮抗菌均可产生蛋白酶、纤维素酶以及果胶酶。为刺梨白粉病的生物防治提供了理论基础,具有进一步研究价值。
In order to screen out the antagonistic bacteria that have a good inhibitory effect on Rosa roxburghii Tratt powdery mildew disease,from healthy Rosa roxburghii Tratt and its root soil,Sphaerotheca pannosa was used as a target strain and the antagonistic bacteria were treated with leaf disk method.the antagonistic bacteria were identified by morphological,physiological and biochemical characteristics,bacterial 16 s rRNA. And by measuring the OD value antagonistic bacteria draw the growth curve.Exploring the antagonistic effect on powdery mildew spore germination and mycelium and enzyme production.The results showed that 48 strains of bacteria were isolated,which JK-6 and JK-32 had significant inhibitory activity,identified as Bacillus amyloliquefaciens and Bacillus aerius,and the control effect reached 60% and 57%. At the same time,under the action of antagonistic bacteria,the spores of pathogenic bacteria caused abnormal swelling during germination,and the surface of the mycelium became rough,swollen,entangled and fused.Enzyme production showed that both antagonistic bacteria could produce protease,cellulase and pectinase.It provided the theoretical basis for the biological control of powdery mildew of prickly pear and had further research value.
作者
冉继平
闫岩
王明力
李岑
万良玉
RAN Ji-ping;YAN Yan;WANG Ming-li;LI Cen;WAN Liang-yu(The Provincical Key Laboratory of Fermentation Engineering and Biopharmaceutics,Guizhou University,Guiyang 550025,China;School of Food and Wine Engineering,Guizhou University,Guiyang 550025,China;College of Pharmaceutical Engineering,Guizhou Institute of Technology,Guiyang 550003,China)
出处
《食品工业科技》
CAS
CSCD
北大核心
2018年第23期146-154,共9页
Science and Technology of Food Industry
基金
贵州省科技合作计划(黔科合LH字[2015]7099)
关键词
刺梨白粉病
拮抗细菌
筛选
Rosa roxburghii Tratt powdery mildew disease
antagonistic bacteria
screening
