摘要
目的探讨血清长链非编码RNA(long non-coding RNA,LncRNA) LIPCAR在冠心病和冠心病合并抑郁患者血清中的表达水平,分析LncRNA LIPCAR在冠心病并抑郁及与临床特征的关系。方法选取冠心病并抑郁患者(合并组)、冠心病患者(冠心病组)各40例,选取健康体检者80例作为对照组,均抽取空腹静脉血后离心取血清,提取血清中的总RNA,反转录获得c DNA后通过qRT-PCR方法检测LncRNA LIPCAR的表达量,分析LncRNA LIPCAR与冠心病、冠心病并抑郁临床特征的关系,分析LncRNA LIPCAR在冠心病并抑郁患者中与临床指标关系,分析LncRNA LIPCAR的表达与Gensini评分、抑郁自评量表(self-rating depression scale,SDS)及汉密尔顿抑郁量表(Hamilton depression scale,HAMD)评分的相关性。结果 3组的LncRNA LIPCAR的表达量组间对比差均有统计学意义(P <0. 05),其中合并组的血清LncRNA LIPCAR的表达量显著高于冠心病组和对照组,差异均有统计学意义(P <0. 05),合并组急性心肌梗死(acute myocardial infarction,AMI)、不稳定性心绞痛(unstable angina pectoris,UAP)和稳定性心绞痛(stable angina pectoris,SAP)三种类型患者的LncRNA LIPCAR水平均显著高于冠心病组,差异有统计学意义(P <0. 05),其中LncRNA LIPCAR表达水平与高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)呈显著负相关(r=-0. 212,P=0. 045),与Gensini评分、SDS评分及HAMD评分均呈显著正相关(r=0. 372、0. 428、0. 442,P <0. 001)。结论血清LncRNA LIPCAR在冠心病合并抑郁症患者中表达显著升高,其与冠心病及抑郁症的严重程度均呈显著正相关关系。
Objective To investigate the expression level of long non-coding RNA(LncRNA)LIPCAR in the serum of patients with coronary heart disease and patients with coronary heart disease combined with depression,and to analyze the correlations between LncRNA LIPCAR and patient's clinical characteristics.MethodsForty patients with coronary heart disease and depression(combined group),40patients with coronary heart disease(CHD group)and80 healthy subjects(control group)were selected.Fasting venous blood was collected.The serum was extracted by centrifugation and the total RNA was extracted from the serum.The expression of LncRNA LIPCAR was assessed by real rt- PCR.The correlations between LncRNA LIPCAR and clinical features of patients of coronary heart disease combined with depression were analyzed.The correlations between LncRNA LIPCAR expression and Gensini score,self-rating depression scale(SDS)and Hamilton depression scale(HAMD)scores were also analyzed.ResultsThe expression differences of LncRNA LIPCAR among the three groups were statistically significant(P<0.05).The expression level of Ln-cRNA LIPCAR in the combined group was significantly higher than those in the CHD group and the control group(P< 0.05).The levels of LncRNA LIPCAR in patients with acute myocardial infarction(AMI),unstable angina pectoris (UAP)and stable angina pectoris(SAP)in the combined group were significantly higher than those in the CHD group (P<0.05).The LncRNA LIPCAR expression level was significantly correlated with high density lipoprotein cholesterol (HDL-C)(r=-0.212,P=0.045).There were also significant positive correlation between LncRNA LIPCAR and Gensini score,SDS score and HAMD score(r=0.372,0.428and0.442,P<0.001).ConclusionThe expression ofLncRNA LIPCAR in patients with coronary heart disease complicated with depression is significantly increased,which is positively correlated with the severity of coronary heart disease and depression.
作者
余天浩
李瑜辉
伍灏堃
杨瑶琳
廖文君
李建云
陈森
YU Tian-hao;LIYu-hui;WUHao-kun;YANGYao-lin;LIAO Wen-jun;LIJian-yun;CHEN Sen(Department of Cardiovascular Diseases,Guangdong Second Provincial General Hospital,Guangzhou510317,Guangdong,China)
出处
《广东医学》
CAS
2018年第21期3182-3186,共5页
Guangdong Medical Journal
基金
广东省医学科研基金资助项目(编号:A2017242)
