摘要
目的:探讨槲皮素对人急性B淋巴细胞白血病(B-ALL)NOD/SCID小鼠的细胞周期及黏附分子的影响。方法:对48只NOD/SCID小鼠于尾静脉注射5×106 Nalm-6细胞,建立ALL NOD/SCID小鼠模型,模型15 d后将ALL NOD/SCID小鼠随机分为3组:对照组(腹腔注射生理盐水(0.2 ml/只),环磷酰胺组(腹腔注射环磷酰胺100μg/kg)及槲皮素组(腹腔注射槲皮素3 mg/kg),治疗21 d后用流式细胞术检测Nalm-6细胞在G1、G2、M和S周期细胞百分率;计数治疗前后全血B淋巴细胞、Nalm-6细胞、中性粒细胞百分率及白细胞总数;采用双抗体夹心法测定治疗前后细胞间粘附分子-1(ICAM-1)、血管细胞粘附分子-1(VCAM-1)和P-选择素(P-selectin)蛋白表达。结果:与治疗前比较,治疗后槲皮素组ICAM-1、VCAM-1和P-selectin蛋白表达降低。血象显示,外周血中性粒细胞明显升高,而B淋巴细胞、白细胞和Nalm-6细胞明显降低; G0/G1期细胞增殖比例减少,S期和G2-M增多(P <0.05)。结论:槲皮素可能通过抑制ICAM-1蛋白表达而降低细胞间的粘附性,并将Nalm-6细胞阻滞在S期和G2-M期,对急性淋巴细胞白血病有明显的抑制作用。
Objective: To investigate the effect of Quercetin on cell cycle and adhesive molecules of NOD.SCID mice with acule B lymphocytic leuaemia(B-ALL).Methods: 5×10^6 Nalm-6(B-ALL cell line) cells were injected into the tail vein of 48 NOD/SCID mice to establish the NOD/SCID mice with B-ALL.After 15 day, the NOD/SCID mice with B-ALL were randomly divided into 3 groups: salive group as control (injection with saline of 0.2 ml/mouse), cyclophos-phamid group (injection with cyclophosphamide of 100 μg/kg) and quercetin group(injection with quercetin of 3 mg/kg).After treatment for 21 d, the perecntage of Nalm-6 cells in G1, G2, M and S phases was detected by flow cytonetry; the B lymphocytes Nalm- 6 cells, neutrophils and WBC in while blood were counted before and after treatment; the expression of intercellalar.Adhesion molecole-1(FCAIU-1), vascular cell adhesion molecule-1(VCAM-1) and P-selectin was detected by double autibody soundwich method.Results: Compared with level before treatment, the expression of ICAM-1, VCAM-1 and P-selectin decreased after treatment with guercetin, The hemogram showed that the peripheral blood nentrophil level obviously increased, while the levels of B lymphocytes, Nalm-6 cells and WBC count decreased obviously after treatment with guercetin.The cell proliferatim rario in G0/G1 phase decreased, yet the cell proliferation ratio in S and G2/M phases increased after treatment with guercetin.Conclusion: The guercetin can decrease the intercellular adhesion through inhibition of ICAM-1 expression, and arrests Nalm-6 cells in S and G2/M phases.The guercetin has obviously inhibitory effect on B-ALL cells.
作者
王黎
戴红卫
郑君
周娇
陈德森
WANG Li;DAI Hong-Wei;ZHENG Jun;ZHOU Jiao;CHEN De-Sen(Depertment of Hematology,The Affiliated People's Hospital of Hubei Medical and Pharmaceutical College,Shiyan 442000,Hubei Province,China;Department of Blood Trausfusion,Suizhou Hospital Affiliated to Hubei Medical and Pharmaceutical College,Shuizou 441300,Hubei Province,China;School of Basic Medicine,Hubei Medical and Pharmaceutical College,Shiyan 442000,Hubei Province,China)
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2018年第6期1616-1620,共5页
Journal of Experimental Hematology
基金
湖北省卫生厅2016自然科学基金指导性项目(2016CFC762)
