摘要
目的:探究蒙药那仁满都拉对系膜增生性肾小球肾炎大鼠尿液PRO、BIL血清IL-6、TNF-α及肾脏病理改变的影响。方法:56只Wistar雄性大鼠随机分为正常组和模型组,模型组大鼠采用邹氏改良的慢性血清病大鼠模型的方法复制系膜增生性肾小球肾炎模型,取32只造模成功的大鼠,随机分为模型组、那仁满都拉低、高剂量组和六味地黄丸组,每组8只。那仁满都拉低、高剂量组分别按1.5 g/(kg·d)、4.5 g/(kg/d)灌胃;六味地黄丸组按1.0 g/(kg·d)灌胃;正常组和模型组灌服等量蒸馏水,连续给药3周。3周后检测各组大鼠尿液PRO、BIL的含量;末次给药12 h后麻醉大鼠,腹主动脉取血,测定血清IL-6、TNF-α含量;处死大鼠取右肾脏组织进行HE染色,在光学显微镜下观察其病理变化。结果:那仁满都拉低、高剂量组尿液PRO含量和血清IL-6和TNF-α的含量均低于模型组(P<0.01或P<0.05),那仁满都拉高剂量组尿液BIL含量低于模型组(P<0.05);那仁满都拉各给药组随剂量增大肾脏组织的病理改变减轻,肾小球系膜细胞增生减轻,炎细胞浸润、蛋白管型、细胞管型、玻璃样变和空泡变逐渐减少。结论:蒙药那仁满都拉可降低系膜增生性肾小球肾炎大鼠血清IL-6、TNF-α及尿液PRO、BIL含量,减轻大鼠肾脏病理改变。
Objective:To study the therapeutic effect of Mongolian medicine Na Ren Man Du La on mesangial proliferative glomerulonephritis.Methods:A total of 56 wistar male rat randomly divided into normal group and model group.In the model group,the model of mesangial proliferative glomerulonephritis was established by Zou's modified chronic serum disease model.A total of 32 model rats were randomly divided into model group,Na Ren Man Du La low dose group,Na Ren Man Du La high dose group,Liuwei Dihuang wan (六味地黄丸)group,8 in each group.Treatment group were respectively intragastric administration at 1.5,4.5 and 1.0g/(kg·d),rats in the normal and model groups were intragastric administration by equal volume of distilled water,take the rats urine from each group after intragastric administration with 3 consecutive week,detect the content of PRO,BIL by urine analyzer;The rats were anesthetized and taken the blood from aorta abdominalis after 12h later than last intragastric administration and fasting,detect the content of IL-6and TNF-α in serum;The pathological changes were observed under light microscope through HE staining after sacrificed the rats and taken the renal tissue from right kidney.Resuhs:Na Ren Man Du La low dose group and high dose group could significantly decreased the content of urine PRO and the content of IL-6TNF-α in serum (P<0.01or P<0.05),Na Ren Man Du La high dose group significantly decreased the content of urine BIL (P<0.05);Na Ren Man Du La each dose group reduce the pathological changes like Mesangial cell hyperplasia,Inflammatory cell infiltration,Cell tube type,Glass-like changes and cavitation changes following the increased of dose of Na Ren Man Du La.Conclusion:Mongolian medicine Na Ren Man Du La has the therapeutic effect on mesangial proliferative glomerulonephritis.The mechanism probably related to the regulation of IL-6and TNF-α and other cytokines.
作者
陈红梅
敖道夫
王秀兰
靳文杰
吴凤娇
CHEN Hong-mei;AO Dao-fu;WANG Xiu-lan;JIN Wen-jie;WU Feng-jiao(Aftiliated Hospital of Inner Mongolia University for the Nationalities,Tongliao Inner Mongolia 028000,China;Mongolia Medical Department of Inner Mongolia University for the Nationalities,Tongliao Inner Mongolia 028000,China)
出处
《中医药导报》
2018年第19期91-93,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(81641149)
关键词
蒙药那仁满都拉
六味地黄丸
系膜增生性肾小球肾炎
大鼠
mongolian medicine Na Ren Man Du La
Liuwei Dihuang wan
mesangial proliferative glomeru- lonephritis
rats
