摘要
目的:探讨LncRNA PRNCR1(prostate cancer non-coding RNA 1)相关的4个基因多态性位点与胃癌发病风险及病理特征的相关性。方法:研究纳入300例经病理确诊的胃癌患者和300例健康者进行病例对照研究;基因分型采用Mass-array基因分析平台完成;采用酶联免疫吸附实验(ELISA)检测幽门螺杆菌(H. pylori)感染状态。结果:LncRNA PRNCR1 rs16901946位点的基因型分布在对照组及病例组中有显著性差异(P=0.018)。与野生型AA基因型相比,rs16901946位点AG基因型(AG vs. AA:校正后OR=1.41,95%CI:1.00～1.98,P=0.048)、GG基因型(GG vs. AA:校正后OR=2.57,95%CI:1.15～5.75,P=0.022)及AG/GG基因型(AG/GG vs. AA:校正后OR=1.51,95%CI:1.09～2.09,P=0.014)的胃癌相对发病风险较高。亚组分析显示,该位点在男性(AG/GG vs. AA:校正后OR=1.82,95%CI:1.23～2.69,P=0.003)、在H. pylori感染人群(AG/GG vs. AA:校正后OR=1.83,95%CI:1.16～2.89,P=0.009)发病风险相对较高。对肿瘤病理特征进行亚组分析显示,该位点与贲门癌的发病风险相关(AG/GG vs. AA:校正后OR=1.58,95%CI:1.10～2.28,P=0.011)、早期胃癌风险相关(AG/GG vs. AA:校正后OR=1.88,95%CI:1.18～2.98,P=0.008)。结论:lncRNA PRNCR1 rs16901946的遗传多态性位点与胃癌发病风险相关,在男性及H. pylori感染阳性人群中的相对风险高。
Objective:To investigate the susceptibility of four genetic variations of prostate cancer non-coding RNA 1(LncRNA PRNCR1)to the risk and pathological characteristics of gastric cancer.Methods:A total of 300gastric cancer cases and 300health controls were enrolled in this case-control study.The Mass-array gene analysis platform was applied to genotype the enrolled participants.Enzyme-linked immunosorbent assay was used to detect the H.pylori infection.Results:There were significant differences of LncRNA PRNCR I rsi6901946genotypes distribution between the cases and the control group (P=0.018),and the AG (ORadjusted=1.41,95%CI:1.00-1.98,P=0.048),GG (ORadjusted=2.57,95%CI:1.15-5.75,P=0.022)and AG/GG genotype (ORadjusted=1.51, 95%CI:1.09-2.09,P=0.014)were associated with increased risk of gastric cancer,respectively.The results of subgroup analysis showed that LncRNA PRNCR1 rs16901946was associated with the risk of gastric cancer in the subgroup of male (AG/GG vs.AA: ORadjusted=1.82,95%CI:1.23-2.69,P=0.003),and individuals with H.pylori infection (AG/GG vs.AA:ORadjusted=1.83,95%CI:1.16-2.89,P=0.009).In addition,LncRNA PRNCR1rs16901946was associated with risk of cardia cancer (AG/GG vs.AA:ORadjusted=1.58, 95%CI:1.10-2.28,P=0.011).Similarly it was associated with risk of gastric cancer in clinical stage T1-T2(AG/GG vs.AA:ORadjusted=1.88,95%CI:1.18-2.98,P=0.008)but not in T3-T4.Conclusion:This study indicates that LneRNA PRNCR1rs16901946is associated with the increased risk of gastric cancer,especially for male and individuals with H.pylori infection.
作者
杨小兵
王劲松
周琎
潘超
Yang Xiaobing;Wang Jingsong;Zhou Jin;Pan Chao(Department of Pathology,Nanjing Hospital Affiliated to NMU,Nanjing 210006,China;Department of General Surgery,Nanjing Hospital Affiliated to NMU,Nanjing 210006,China)
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第11期1520-1524,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
南京医科大学科技发展基金重点项目(2017NJMUZD097)。
