摘要
目的:探讨丙烯酰胺(ACR)暴露对Wistar大鼠学习记忆能力、大脑海马CA1区c-Fos蛋白和c-fos mRNA表达的影响。方法:健康雄性Wistar大鼠40只,体重180~220g,随机分为对照组、腹腔注射ACR9mg/kg组、18mg/kg组和36mg/kg组,每组各8只,对照组以同样方式腹腔注射等量生理盐水。1周后用Morris水迷宫法检测大鼠学习记忆功能,HE染色观察海马CA1区神经细胞形态,用免疫组化法检测海马CA1区c-Fos蛋白表达,RT-PCR法检测c-fos基因表达。结果:ACR组大鼠表现为海马CA1区神经纤维稀疏、神经细胞减少,可见空泡样变性等。与对照组比较,ACR作用后大鼠学习记忆功能明显降低(P<0.05),且具有剂量依赖性(r=0.820~0.891,P均<0.05)。ACR中、高剂量组海马区c-Fos蛋白和c-fosmRNA表达水平明显低于对照组(P<0.01),同时随ACR剂量增加,c-Fos蛋白和c-fosmRNA表达水平降低,与ACR剂量明显相关(r=-0.824,0.857,P均<0.05)。结论:模型大鼠暴露于ACR可以损害学习记忆功能,同时有海马区c-Fos蛋白和c-fosmRNA表达水平下降,其机制可能是ACR造成大鼠海马区神经细胞功能减弱所致。
Objective: To investigate the effects of acrylamide ( ACR) exposure on learning and memory ability, expressions of c-Fos protein and c-fos mRNA in CA1 area of hippocampus in Wistar rats.Methods: Forty healthy male Wistar rats weighing 180 ~ 220 g were randomly divided into control group,intraperitoneal injection of ACR 9 mg /kg group,18 mg /kg group and 36 mg /kg group.Each group was 8 rats,while the control group received intraperitoneal injection of the same amount of normal saline in the same way.After 1 week,the learning and memory function of the rats was detected by Morris water maze.The morphology of neurons in hippocampal CA1 area was observed by HE staining.The expression of c-Fos protein in hippocampal CA1 area was detected by immunohistochemistry,and the expression of c-fos gene was detected by RT-PCR.Results: In ACR group,the nerve fibers in CA1 area of hippocampus were sparse, the nerve cells were decreased and vacuole-like degeneration was observed.Compared with the control group,the learning and memory function of the rats after exposure to ACR was significantly lower ( P<0.05) ,and had a dose effect correlation ( r = 0.820 - 0.891,P<0.05) .The expression levels of c-Fos protein and c-fos mRNA in hippocampus of ACR middle and high dose group were significantly lower than those of control group ( P<0.01) .At the same time,the expression of c-Fos protein and c-fos mRNA decreased with the increase of ACR dose,which was negatively correlated with ACR dose ( r = - 0.824,0.857,P<0.05) .Conclusion: The exposure to ACR can damage the learning and memory function in the rats,while the expression level of c-Fos protein and c-fos mRNA decreases in hippocampal CA1 area.The mechanism may be that ACR causes the weakening of neurons in the hippocampus of rats by ACR.
作者
方桦
刘合玉
朱宁
赵钢
岳屹立
Fang Hua;Liu Heyu;Zhu Ning;Zhao Gang;Yue Yili(Department of Neurological Rehabilitation,Second Affiliated Hospital,Zhengzhou University,Zhengzhou 450000;Department of Neurology,Xijing Hospital,Fourth Military Medical University,Xi'an 710032;Department of Pathophysiology,Medical College of Yan'an University,Yan'an 716000,China)
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2018年第6期699-704,共6页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金(30770670)
