摘要
目的:研究党参甲醇提取物中的化学成分,并对分离获得的化学成分进行体外抗氧化活性检测。方法:利用D101型大孔吸附树脂,MCI树脂,硅胶,十八烷基硅烷键合硅胶(ODS),羟丙基葡聚糖凝胶(Sephadex) LH-20等柱色谱及制备硅胶薄层色谱进行化合物分离,采用核磁共振(NMR)和质谱(MS)等方法进行化合物结构鉴定;采用1,1-二苯基-2-三硝基苯肼(DPPH),2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)和超氧阴离子自由基清除能力检测各化合物的抗氧化活性,以维生素C为阳性对照。结果:从党参的甲醇提取物中分离得到7个化合物,分别鉴定为7R/7S,8S-guaiacylglycerol-8-O-4’-(coniferyl alcohol) ether(1/2),党参炔苷(3),苍术内酯Ⅲ(4),紫丁香苷(5),党参苷Ⅰ(6),党参吡咯烷鎓B(7)。化合物1和2具有一定的DPPH和ABTS+·自由基清除能力,而化合物1~7对O-2·自由基的清除能力较弱。结论:化合物1和2为木脂素类中的新木脂素结构类型,为首次从党参属中分离得到,并具有一定的体外抗氧化活性。
Objective:To study on the chemical constituents in Codonopsis Radix,and to investigate their in vitro antioxidant activities of isolated compounds.Method:The chemical constituents were isolated and purified by D101macroporous resin,MCI resin,silica gel,oetadecyl silane bonded silica (ODS),Sephadex LH-20column chromatography and preparative silica gel thin layer chromatography.Nuclear magnetic resonance (NMR)and mass spectrometry (MS)were used to elucidate the structures of obtained compounds.The antioxidant effects of isolated compounds were evaluated by radical scavenging ability of 1,1-diphenyl-2- picrylhydrazyl (DPPH),2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS)and superoxide anion with vitamin C as positive control.Result:Seven compounds were obtained and identified as 7R/7S,8S- guaiacylglycerol-8-O-4'-(coniferyl alcohol)ether (1/2),lobetyolin (3),atractylenolide IlI (4),syringin (5),tangshenoside I (6),eodonopyrrolidium B (7).Among them,compound 1and 2showed potential capacity for DPPH and ABTS^+·radical scavenging,compound 1-7showed relatively low capacity for 0^-2·radical scavenging.Conclusion:Compound 1and 2,identified as neolignans;are isolated from the genus Codonopsis for the first time and exhibit certain in vitro antioxidant activities.
作者
张鑫
李建宽
赵玉静
高建平
ZHANG Xin;LI Jian-kuan;ZHAO Yu-jing;GA0Jian-ping(School of Pharmacy,Shanxi Medical University,Taiyuan 030001,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第24期53-59,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
山西省科技厅科技攻关项目——振东专项(2014ZD0404)
山西省重点研发计划重点项目(201603D3111005)
国家中药标准化项目(ZYBZH-Y-JIN-34)
山西省道地药材资源开发工程技术研究中心项目
2016年山西省重点学科建设项目(FSKSC)
关键词
党参
化学成分
木脂素
柱色谱
抗氧化活性
羟丙基葡聚糖凝胶
甲醇提取物
Codonopsis Radix
chemical constituents
lignan
column chromatography
antioxidant activity
Sephadex
methanol extract
