摘要
目的:探讨皖南地区眼镜蛇毒活性组分(cobra venom analgesic factor,CVAF)外用镇痛的效应及其可能的作用机制。方法:雄性SD大鼠分为生理盐水对照组(NS组)、完全弗氏佐剂炎痛组(CFA组)、双氯芬酸钠镇痛阳性对照组(CFA+SL组)、氟比洛芬镇痛阳性对照组(CFA+FB组)、CVAF镇痛实验组(CFA+CVAF组),比较观察CVAF凝胶局部外用的镇痛效果。分别采用热刺痛仪及机械压痛仪测定各组大鼠痛阈,ELISA法检测各组大鼠脊髓匀浆中IL-1、TNF-α的含量,HE染色法观察大鼠足趾组织病理变化。结果:与CFA组比较,CVAF镇痛组大鼠在给药后的第7日和第14日的体质量差异具有统计学意义(P <0. 05、P <0. 01),CVAF组大鼠的机械压痛阈和热痛阈都有所提高(P <0. 01),脊髓匀浆中IL-1、TNF-α的浓度相对减少,差异有统计学意义(P <0. 01),在HE染色中,CVAF组大鼠足趾组织水肿明显减轻,炎性浸润相对不显著。结论:皖南地区CVAF凝胶局部外用可提高炎痛大鼠热痛阈和机械压痛阈,其机制可能与减少炎症介质释放,抑制炎症反应有关。
AIM: To explore the effect of topical analgesia of cobra venom analgesic factor (CVAF) in southern Anhui province and its possible mechanism. METHODS: Male SD rats were divided into normal saline control group (NS group), complete Freund's adjuvant pain group (CFA group), diclofenac sodium analgesic positive control group (CFA+SL group), flurbiprofen analgesic positive control group (CFA+FB group), CVAF analgesia group (CFA+CVAF group). The analgesic effect of CVAF gel, the active component of cobra venom was observed and compared. The pain thresholds of rats in each group were measured with a thermal tingling tester and mechanical tenderness tester. The content of IL-1 and TNF-α in the homogenate of spinal cord in each group was detected by using ELISA, the pathological changes of rat toe tissue was observed by using HE staining.RESULTS:Compared with CFA group, the weight loss of CVAF analgesia rats on the 7th and 14th day after administration was improved (P<0.05, P<0.01). The mechanical pain threshold and the thermal pain threshold of the CVAF group were all improved (P<0.01). The concentrations of IL-1 and TNF-α in the homogenate of spinal cord were relatively low (P<0.01). In HE staining, the toe tissue edema in the CVAF group was significantly reduced, and the inflammatory infiltration was relatively insignificant. CONCLUSION: The CVAF gel, the active ingredient of Cobra venom in southern Anhui province, can improve the inflammatory pain in rats hot pain threshold and mechanical pressure threshold; its mechanism may be related to reduce the release of inflammatory mediators, inhibiting the inflammatory response.
作者
俞玲
张根葆
孙淑萍
YU Ling;ZHANG Genbao;SUN Shuping(Department of Pathophysiology,Wannan Medical College,Wuhu 241001,Anhui,China)
出处
《中国临床药理学与治疗学》
CAS
CSCD
2018年第11期1235-1239,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
安徽省高校省级自然科学研究重点项目(KJ2011A266)
关键词
眼镜蛇毒
凝胶剂
镇痛
抗炎
cobra venom
gel preparation
analgesia
anti-inflammatory
