摘要
【目的】探索酿酒葡萄果实性状的遗传规律,提高酿酒葡萄杂交育种效率。【方法】以毛欧杂种'2-1-3'为母本的2个酿酒葡萄杂交组合69个F1代成熟果实为材料,分析了果实可溶性固形物、可滴定酸和酚类物质含量等21项指标,并进行遗传倾向分析。【结果】'2-1-3'×'阿列尼'、'2-1-3'×'宝石解百纳'F1代果实性状均为数量性状,呈连续分布;果粒质量平均值稍高于亲中值,有超高亲子代,但介于双亲之间的比例大;子代可溶性固形物含量均值高于亲中值,'2-1-3'×'阿列尼'F1代多居于亲本之间,而'2-1-3'×'宝石解百纳'F1代超高亲比例大;子代可滴定酸含量均值低于亲中值,超低亲比例大。子代果皮总花色苷含量均值低于含量低的亲本,超低亲比例大。'2-1-3'×'阿列尼'F1代果皮原花色素、果肉总酚、总类黄酮和原花色素含量均值均高于亲中值,超高亲比例大;其余酚类物质指标均值均低于亲中值,种子酚类物质介于亲本之间比例较大,其余4个指标超低亲比例大。'2-1-3'×'宝石解百纳'F1代果皮酚类物质含量、果肉总酚、单宁和原花色素含量均值均低于亲中值,果肉原花色素介于亲本之间的比例最大,其余指标超低亲比例大;果肉和种子其余酚类物质指标均值均大于亲中值,介于亲本之间的比例大。【结论】组合类型不同,性状遗传倾向有差异。
[Objective]Breeding new grape varieties is very important for promoting grape industry.In order to explore the genetic laws of fruit traits in wine grapes and to improve predictability and breeding efficiency of grape crossbreeding,we researched the genetic tendency and analyzed the inheritance of the fruit traits of 69 hybrid plants generated from hybridizing‘2-1-3’(Vitis quinquangularis Rehd.×Vitis vinifera)with 2 wine grape varieties.[Methods]2 crossing combinations hybridization was made using‘2-1-3'as female parent in 2007 and 69 F1 progenies were obtained.The hybrid plants bore berries in 2012.The berries were collected from grape vines grown in the vineyards of Pomology Institute,Shanxi Academy of Agricultural Science in Taigu,Shanxi province during August to October in 4 years(2013-2016).The fruit quality indexes of berry weight,the ratio of peel and pulp,soluble solids(SS),titratable acid(TA)content,pH value,juice yield in fresh fruits were measured according to the conventional method.The samples were frozen using liquid nitrogen and stored in an ultra-low temperatu freezer.The contents of phenolic compounds in skin,pulp and seeds of berries were analyzed for 4 years according to colorimetric method.The data were processed by Excel statistical software,and the genetic tendency of fruit characteristics were analyzed by the indexes of coefficient variation(CV),heritability(Ta),heterotic rate.[Results]In the crossing combinations of‘2-1-3'בAreni'and‘2-1-3’בRuby Cabernet’,the fruit characteristics of F1 progeny were quantitative traits with continuous distribution,the value of CV in the 21 fruit indexes was 3.18%-142.98%,6.87%-109.75%,respectively,especially the value of CV of the fruit shape index,pH value and juice yield were below 10%,while the value of CV the Ta was 50.18%-235.89%,73.77%-146.0%,respectively.The average berry weight of F1 progenies was slightly above the mid-parent value,there were some ultra high individuals,but more than half progeny had mid berry weight between parents'value(56.67%,73.81% respectively in the crossing combinations of‘2-1-3’בAreni’and‘2-1-3’בRuby Cabernet’).The SS content of F1 progenies was higher than the mid-parent value,the proportion of progeny which had mid SS content between parents'value in the crossing combination of‘2-1-3’בAreni’was more the half(51.85% ),while the proportion of progeny which has SS content higher than parents in the crossing combination of‘2-1-3’בRuby Cabernet’was largest(47.62%).The TA content of F1 progenies was lower than the mid-parent value,and the proportion of progeny which had TA content lower than parents'value was 51.85%,50.0% respectively in the crossing combinations of‘2-1-3’בAreni’and‘2-1-3’בRuby Cabernet’.The average total anthocyanin content of F1 progenies was lower than that of the parent with low content,and the proportion of progeny which had lower content than parents'value was larger than 60%.In the crossing combination of‘2-1-3’בAreni’,the mean values of proanthocyanidins content in the skin,total phenols,total flavonoids,and proanthocyanidins content in the pulp of F1 progenies were higher than their mid-parent values,and the proportion of progeny which had higher content than parents'value was 48.15%,44.44%,55.56%,55.56%,respectively;the average value of the other phenolic indicators was lower than their mid-parent values,the proportion of progeny which had the phenolic content between the parents in the seeds was relatively larger,however,the proportion of progeny which has the phenolic content below the parents in the remaining 4 indicators was larger.In the crossing combination of‘2-1-3’בRuby Cabernet’,the content of phenolic compounds in skin,total phenolics,tannins and proanthocyanidin content in pulp of the progeny was lower than the mid-parent value,and the proportion of progeny which had the proanthocyanidin content in pulp between the parents was the largest,however,the proportion of progeny which had the lower content in the other indicators than the parents was the lowest;the average content of the other phenolic compounds in pulp and seeds was higher than the mid-parent value,and the proportion of progeny which had contents between the parents was the largest.[Conclusion]The mean value of berry weight in the two crossing combinations was slightly higher than the mid-parent value,and there had emergence of super-high progeny.The average SS content of F1 progenies was higher than the mid-parent value;in addition to the additive effect of the genes,there was a certain non-additive effect and a certain heterosis.The mean TA content of F1 progenies was lower than the mid-parent value,tending towards the low acid parent.The genetic tendencies of the two crossing combinations in phenolic compounds were somehow different.This may be due to the difference in the combination type and the difference in the genetic tendency of the traits.
作者
谭伟
李晓梅
董志刚
谭敏
杨兆亮
唐晓萍
TANWe;LI Xiaomei;DONG Zhigang;TAN Min;YANG Zhaoliang;TANG Xiaoping(Pomology Institute,Shanxi Academy of Agricultural Science.Shanxi Key Laboratory of Germplasm Improvement and Utilization in Pomology,Taigu 030815,Shanxi,China;College of Food Science and Pharmaceutical Engineering,Zaozhuang University,Zaozhuang 277160,Shandong,China)
出处
《果树学报》
CAS
CSCD
北大核心
2018年第12期1444-1454,共11页
Journal of Fruit Science
基金
现代农业产业技术体系葡萄专项资金(CARS-29-yc-5)
山西省应用基础研究面上青年基金(201601D202067)
山西省农业科学院院优势课题组自选项目(YYS1704)
山西省农业科学院博士研究基金(YBSJJ1308)
山西省重点研发计划重点项目(201603D21105)
关键词
酿酒葡萄
品种
杂交组合
果实性状
遗传倾向
Wine grape
Variety
Hybrid progeny
Fruit traits
Inheritance trend
