摘要
目的:研究我国北方汉族肥胖症患者黑皮质素受体4(melanocortin-4 receptor,MC4R)基因的rs17782313位点基因多态性对腹腔镜Roux-en-Y胃旁路术(1aparoscopic Roux-en-Y gastric bypass,LRYGB)减重效果的影响。方法:回顾性分析中国医科大学附属盛京医院2011年1月至2013年12月收治的81例病态性肥胖接受LRYGB治疗的患者临床资料,提取患者外周血DNA,PCR扩增目的基因后应用直接测序法,对rs17782313位点基因型进行检测。根据基因型分组:风险基因携带组和非携带组,比较分析2组代谢指标、体质指数(body mass index,BMI),多余体质量减少率(excess weight loss,EWL)等术后变化,评价该基因对减重效果的影响。结果:(1)rs17782313位点的3种基因型TT、TC、CC,基因频率分别为50.62%(41例)、35.80%(29例)、13.58%(11例)。(2)不同基因型的各组间,体质量、BMI、血脂、空腹血糖、糖化血红蛋白等指标无明显差异。(3)风险基因携带者术后BMI、EWL下降少于非携带者,但未达到统计学差异(女性患者BMI术后6个月P=0.296,12个月P=0.179;EWL P=0.139,0.094。男性患者BMI术后6个月P=0.295,12个月P=0.072;EWL P=0.106,0.077)。结论:MC4R基因rs17792313位点C等位基因(风险基因)携带者与非携带者的减重效果相似。
Objective:To investigate the influence of melanocortin-4 receptor(MC4R)gene rs17782313 polymorphism on the weight-reducing effect of laparoscopic Roux-en-Y gastric bypass(LRYGB). Methods:A retrospective analysis was performed for the clinicaldata of 81 patients with morbid obesity who were admitted to Shengjing Hospital of China Medical University from January 2011 toDecember 2013 and underwent LRYGB. Peripheral blood DNA was extracted,the target gene was amplified by PCR,and then directsequencing was used to determine the genotype of rs17782313. According to the genotype,the patients were divided into risk genegroup and non-risk gene group,and the two groups were compared in terms of the changes in metabolic indices,body mass index(BMI),and excess weight loss(EWL)after surgery. Results:The frequencies of TT,TC,and CC genotypes of rs17782313 were 50.62%(41 patients),35.80%(29 patients),and 13.58%(11 patients),respectively. There were no significant differences between the patientswith different genotypes in body weight,BMI,blood lipids,fasting blood glucose,and glycosylated hemoglobin. The non-risk genegroup had slightly greater reductions in BMI and EWL than the risk gene group at 6 and 12 months after surgery(BMI in female patients:P 6 months =0.296,P 12 months =0.179;EWL in female patients:P 6 months =0.139,P 12 months =0.094;BMI in male patients:P 6 months =0.295,P 12 months =0.072;EWL in male patients:P 6 months =0.106,P 12 months =0.077). Conclusion:The patients who carry C allele(risk gene)in MC4R rs17792313have a similar weight loss as those who do not carry this allele.
作者
刘冰阳
王勇
刘聪
李玲
Liu Bingyang;Wang Yong;Liu Cong;Li Ling(Department of Endocrinology,Shengjing Hospital of China Medical University;Department of General Surgery,No.4Hospital of China Medical University)
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2018年第12期1643-1646,共4页
Journal of Chongqing Medical University
