摘要
膜磷脂是产生胞内信号信使的重要来源,磷脂酶Ds可催化磷脂产生磷脂酸,从而参与信号过程。PLDs是一类重要的磷脂酶家族,拟南芥中已发现了12种PLD,其中10种为C2-PLDs,其生化特性有明显差异。磷脂酶Ds和磷脂酸在不同胁迫下执行相同或独特的细胞信号转导功能:干旱胁迫下PLDα1、PLDδ、PLDα3正向调节ABA信号的应答;盐胁迫下PLDα1、PLDδ、PLDα3、PLDζs和PLDε做出应答;冷冻胁迫下PLDδ-PA和PLDα-PA表现出相反的作用;在磷缺失胁迫下,PLDζ1和PLDζ2正向调节根生长;PLDε在不同氮胁迫水平下应答不同。PLDs的作用机理主要分为三大类:细胞调节、膜脂重组与膜脂降解。其中细胞调节包括信号转导方式,PLD可通过直接作用于蛋白,如与GPA1、GAPC结合;或通过PLDs的产物,如PAs,来应答胁迫。本研究将综述磷脂酶Ds的生化特性,以及在逆境胁迫中的功能与作用机理。
Membrane phospholipid is the important source in generating intracellular signaling messengers.Phospholipase Ds can catalyze phospholipids to produce phosphatidic acid,and thus participate in the signaling process.Phospholipase Ds play important roles in phospholipase family,12 PLD genes are identified in Arabidopsis,10 of which have the N-terminal C2 domain,whose biochemical properties are significantly different.Phospholipase Ds and phosphatidic acids display the overlapping or unique cellular signaling transduction under different stresses.PLDδandpositively regulate the response to ABA signaling in drought stress;PLDδ,and PLDεare in response to salt stress;PLDδ-PA and PLDα-PA play opposite roles in freezing stress;andregulate the growth of roots positively under phosphorus starving;PLDεresponds differently under different nitrogen levels.The mechanism of the specific function of phospholipase Ds mainly contains three categories:cellular regulation,membrane lipid remodeling and degradation.Cellular regulation contains two aspects,phospholipase D acts directly on proteins,such as the combination of GPA1 or GAPC;and indirectly affects proteins by the products of phospholipase Ds,such as phosphatidic acid(PA).The paper summarizes the biochemical property of phospholipase Ds,and its function and mechanism under adversity stress.
作者
王雅静
黄桂荣
张欣莹
钟秀丽
Wang Yajing;Huang Guirong;Zhang Xinying;Zhong Xiuli(Institute of Environment and Sustainable Development in Agriculture,CAAS,Beijing 100081)
出处
《中国农学通报》
2018年第36期31-41,共11页
Chinese Agricultural Science Bulletin
基金
国家重点研发计划课题"化肥减施增效共性技术与评价研究"(2017YFD0201702)
关键词
植物生理学
磷脂酶D
机理
胁迫
磷脂酸
plant physiology
phospholipase D
mechanism
stress
phosphatidic acid
