摘要
目的:探讨牛大力水提物对斑马鱼药物性肝纤维化损伤的作用及其机制。方法:以3月龄野生型AB品系斑马鱼为研究对象,用二乙基亚硝胺(DEN)刺激4周构建斑马鱼药物性肝纤维化模型,后用高、中、低浓度牛大力水提物进行干预。取肝组织做病理HE染色、天狼星红苦味酸染色、I型胶原蛋白(Collagen I)免疫组化染色,检测斑马鱼肝脏匀浆中α-平滑肌激动蛋白(α-SMA)、肿瘤坏死因子-α(TNF-α)、Bcl-2相关X蛋白(Bax)表达水平。结果:与模型组比较,牛大力水提物各给药组斑马鱼肝脏纤维化程度、病理损伤、胶原纤维沉积均有不同程度的减轻,其中以牛大力高浓度组(100 mg/L)效果最为显著;牛大力高浓度组斑马鱼肝脏中Collagen-1、细胞凋亡相关基因α-SMA、TNF-α、Bax的表达均明显下降,差异均有统计学意义(P <0.01)。结论:牛大力水提物对药物性肝纤维化损伤的保护作用是通过抑制肝细胞凋亡、减少胶原纤维沉积实现的。
Objective:To discuss the effect and mechanism of aqueous extract of millettia speciosa champ for drug-induced liver fibrosis in zebrafish. Methods:Selected a 3-month-old wild-type zebrafish in AB strain as a research object, and made a zebrafish model of drug-induced liver fibrosis by stimulating with diethylnitrosamine (DEN) for 4 weeks, followed by intervention of high-, medium- and low-concentrations of aqueous extract of millettia speciosa champ. Taken liver tissue for pathological HE staining,sirius red picric acid staining,and Collagen I(Collagen I) immunohistochemical staining,detected expression levels of α-smooth muscle actin (α-SMA),tumor necrosis factor-α(TNF-α) and Bcl-2-associated X Protein(Bax) in zebrafish liver homogenate. Results:Compared with the model group,the degree of drug-induced liver fibrosis,pathological injury and collagen fiber deposition in each group received administration of aqueous extract of millettia speciosa champ was all reduced to some extent,and the effect was most significant in the group of high-concentration of millettia speciosa champ(100 mg/L);The expressions of Collagen-1 and apoptosis-related genes α-SMA, TNF-α and Bax in the liver of zebrafish in the group of high-concentration of millettia speciosa champ were significantly decreased, difference being significant(P<0.01). Conclusion:The protective effect of aqueous extract of millettia speciosa champ for drug-induced liver fibrosis is achieved by inhibiting hepatocellular apoptosis and reducing collagen fiber deposition.
作者
周楚莹
赖裕玲
谢凌鹏
刘强
高磊
吕志平
ZHOU Chuying;LAI Yuling;XIE Lingpeng;LIU Qiang;GAO Lei;LYU Zhiping
出处
《新中医》
CAS
2018年第12期12-16,共5页
New Chinese Medicine
基金
广州市科技计划项目(201508020014)
关键词
牛大力水提物
药物性肝纤维化
动物实验
斑马鱼
Aqueous extract of millettia speciosa champ
Drug-induced liver fibrosis
Animal experiment
Zebrafish
